2ber
From Proteopedia
(New page: 200px<br /> <applet load="2ber" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ber, resolution 1.80Å" /> '''Y370G ACTIVE SITE M...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BER is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Micromonospora_viridifaciens Micromonospora viridifaciens]] with SLB and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BER OCA]]. | + | 2BER is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Micromonospora_viridifaciens Micromonospora viridifaciens]] with SLB and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BER OCA]]. |
==Reference== | ==Reference== | ||
Structure and mechanism of action of an inverting mutant sialidase., Newstead S, Watson JN, Knoll TL, Bennet AJ, Taylor G, Biochemistry. 2005 Jun 28;44(25):9117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15966735 15966735] | Structure and mechanism of action of an inverting mutant sialidase., Newstead S, Watson JN, Knoll TL, Bennet AJ, Taylor G, Biochemistry. 2005 Jun 28;44(25):9117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15966735 15966735] | ||
| + | [[Category: Exo-alpha-sialidase]] | ||
[[Category: Micromonospora viridifaciens]] | [[Category: Micromonospora viridifaciens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sialidase]] | [[Category: sialidase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:42:20 2007'' |
Revision as of 11:37, 30 October 2007
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Y370G ACTIVE SITE MUTANT OF THE SIALIDASE FROM MICROMONOSPORA VIRIDIFACIENS IN COMPLEX WITH BETA-NEU5AC (SIALIC ACID).
Overview
Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora, viridifaciens sialidase to small amino acids changes the mechanism of, catalysis from retention of anomeric configuration to inversion [Watson, J. N., et al. (2003) Biochemistry 42, 12682-12690]. For the Y370G mutant, enzyme-catalyzed hydrolysis of a series of aryl sialosides and, 3'-sialyllactose, the derived Bronsted parameters (beta(lg)) on k(cat) and, k(cat)/K(m) are -0.63 +/- 0.05 and -0.80 +/- 0.08, respectively. Thus, for, the Y370G enzyme, glycosidic C-O bond cleavage is rate-determining., Analysis of the activity of the Y370G mutant and wild-type enzymes against, a substrate [3,4-dihydro-2H-pyrano[3,2-c]pyridinium, alpha-d-N-acetylneuraminide (DHP-alphaNeu5Ac)] whose hydrolysis cannot be, accelerated by acid ... [(full description)]
About this Structure
2BER is a [Single protein] structure of sequence from [Micromonospora viridifaciens] with SLB and NA as [ligands]. Active as [Exo-alpha-sialidase], with EC number [3.2.1.18]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structure and mechanism of action of an inverting mutant sialidase., Newstead S, Watson JN, Knoll TL, Bennet AJ, Taylor G, Biochemistry. 2005 Jun 28;44(25):9117-22. PMID:15966735
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