1rif

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(New page: 200px<br /><applet load="1rif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rif, resolution 2.&Aring;" /> '''Crystal structure of t...)
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[[Image:1rif.jpg|left|200px]]<br /><applet load="1rif" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1rif.jpg|left|200px]]<br /><applet load="1rif" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1rif, resolution 2.&Aring;" />
caption="1rif, resolution 2.&Aring;" />
'''Crystal structure of the UvsW helicase from Bacteriophage T4'''<br />
'''Crystal structure of the UvsW helicase from Bacteriophage T4'''<br />
==Overview==
==Overview==
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In bacteriophage T4, the WXY system repairs DNA damage by a process that, involves homologous recombination. This system comprises three proteins, the RecA-like recombination protein UvsX, a recombination mediator protein, UvsY, and a helicase UvsW. Here we report the 2.0 A resolution crystal, structure of the N-terminal two domains of the UvsW helicase (UvsWNF;, residues 1-282). The structure reveals a typical helicase RecA-like domain, linked to a small N-terminal alpha/beta domain that likely binds the, nucleic acid substrate. The missing C-terminal portion of UvsW almost, certainly corresponds to the second RecA-like domain typically found in, monomeric helicases. The putative substrate binding domain is unique, within the known helicase structures, and it resembles the novel, "double-wing" DNA binding domain from the phage T4 MotA transcription, factor that mediates the expression of T4 middle genes. The functional, implications of this homology for the role of UvsW in T4 DNA metabolism, are discussed.
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In bacteriophage T4, the WXY system repairs DNA damage by a process that involves homologous recombination. This system comprises three proteins, the RecA-like recombination protein UvsX, a recombination mediator protein UvsY, and a helicase UvsW. Here we report the 2.0 A resolution crystal structure of the N-terminal two domains of the UvsW helicase (UvsWNF; residues 1-282). The structure reveals a typical helicase RecA-like domain linked to a small N-terminal alpha/beta domain that likely binds the nucleic acid substrate. The missing C-terminal portion of UvsW almost certainly corresponds to the second RecA-like domain typically found in monomeric helicases. The putative substrate binding domain is unique within the known helicase structures, and it resembles the novel "double-wing" DNA binding domain from the phage T4 MotA transcription factor that mediates the expression of T4 middle genes. The functional implications of this homology for the role of UvsW in T4 DNA metabolism are discussed.
==About this Structure==
==About this Structure==
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1RIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with MG and AU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RIF OCA].
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1RIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIF OCA].
==Reference==
==Reference==
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[[Category: Bacteriophage t4]]
[[Category: Bacteriophage t4]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Kreuzer, K.N.]]
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[[Category: Kreuzer, K N.]]
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[[Category: Sickmier, E.A.]]
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[[Category: Sickmier, E A.]]
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[[Category: White, S.W.]]
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[[Category: White, S W.]]
[[Category: AU]]
[[Category: AU]]
[[Category: MG]]
[[Category: MG]]
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[[Category: uvsw]]
[[Category: uvsw]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:37:24 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:12 2008''

Revision as of 12:51, 21 February 2008

Image:1rif.jpg

1rif, resolution 2.Å

Drag the structure with the mouse to rotate

Crystal structure of the UvsW helicase from Bacteriophage T4

Overview

In bacteriophage T4, the WXY system repairs DNA damage by a process that involves homologous recombination. This system comprises three proteins, the RecA-like recombination protein UvsX, a recombination mediator protein UvsY, and a helicase UvsW. Here we report the 2.0 A resolution crystal structure of the N-terminal two domains of the UvsW helicase (UvsWNF; residues 1-282). The structure reveals a typical helicase RecA-like domain linked to a small N-terminal alpha/beta domain that likely binds the nucleic acid substrate. The missing C-terminal portion of UvsW almost certainly corresponds to the second RecA-like domain typically found in monomeric helicases. The putative substrate binding domain is unique within the known helicase structures, and it resembles the novel "double-wing" DNA binding domain from the phage T4 MotA transcription factor that mediates the expression of T4 middle genes. The functional implications of this homology for the role of UvsW in T4 DNA metabolism are discussed.

About this Structure

1RIF is a Single protein structure of sequence from Bacteriophage t4 with and as ligands. Full crystallographic information is available from OCA.

Reference

The crystal structure of the UvsW helicase from bacteriophage T4., Sickmier EA, Kreuzer KN, White SW, Structure. 2004 Apr;12(4):583-92. PMID:15062081

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