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3bog
From Proteopedia
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{{Seed}} | {{Seed}} | ||
| - | [[Image:3bog. | + | [[Image:3bog.png|left|200px]] |
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==About this Structure== | ==About this Structure== | ||
| - | 3BOG is a | + | 3BOG is a 4 chains structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOG OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:18598029</ref><references group="xtra"/> | |
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[[Category: Dashnau, J.]] | [[Category: Dashnau, J.]] | ||
[[Category: Gates, Z P.]] | [[Category: Gates, Z P.]] | ||
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[[Category: Sfafp]] | [[Category: Sfafp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:39:50 2009'' |
Revision as of 08:39, 17 February 2009
Snow Flea Antifreeze Protein Quasi-racemate
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the chemically synthesized proteins d-sfAFP and l-sfAFP occurred much more readily than for l-sfAFP alone. More facile crystal formation also occurred from a quasi-racemic mixture of d-sfAFP and l-Se-sfAFP, a chemical protein analogue that contains an additional -SeCH2- moiety at one residue and thus differs slightly from the true enantiomer. Multiple wavelength anomalous dispersion (MAD) phasing from quasi-racemate crystals was then used to determine the X-ray structure of the sfAFP protein molecule. The resulting model was used to solve by molecular replacement the X-ray structure of l-sfAFP to a resolution of 0.98 A. The l-sfAFP molecule is made up of six antiparallel left-handed PPII helixes, stacked in two sets of three, to form a compact brick-like structure with one hydrophilic face and one hydrophobic face. This is a novel experimental protein structure and closely resembles a structural model proposed for sfAFP. These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein.
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3BOG is a 4 chains structure. Full crystallographic information is available from OCA.
Reference
- Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB. X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers. J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029 doi:http://dx.doi.org/10.1021/ja8013538
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