1rqf
From Proteopedia
(New page: 200px<br /><applet load="1rqf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rqf, resolution 2.89Å" /> '''Structure of CK2 bet...) |
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| - | [[Image:1rqf.gif|left|200px]]<br /><applet load="1rqf" size=" | + | [[Image:1rqf.gif|left|200px]]<br /><applet load="1rqf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rqf, resolution 2.89Å" /> | caption="1rqf, resolution 2.89Å" /> | ||
'''Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide'''<br /> | '''Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A truncated form of the regulatory subunit of the protein kinase CK2beta | + | A truncated form of the regulatory subunit of the protein kinase CK2beta (residues 1-178) has been crystallized in the presence of a fragment of the cyclin-dependent kinase inhibitor p21WAF1 (residues 46-65) and the structure solved at 2.9 A resolution by molecular replacement. The core of the CK2beta dimer shows a high structural similarity with that identified in previous structural analyses of the dimer and the holoenzyme. However, the electron density corresponding to the substrate-binding acidic loop (residues 55-64) indicates two conformations that differ from that of the holoenzyme structure [Niefind et al. (2001), EMBO J. 20, 5320-5331]. Difference electron density near the dimerization region in each of the eight protomers in the asymmetric unit is attributed to between one and eight amino-acid residues of a complexed fragment of p21WAF1. This binding site corresponds to the solvent-accessible part of the conserved zinc-finger motif. |
==About this Structure== | ==About this Structure== | ||
| - | 1RQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with ZN and UNK as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 1RQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=UNK:'>UNK</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RQF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Allende, J | + | [[Category: Allende, J E.]] |
[[Category: Bertrand, L.]] | [[Category: Bertrand, L.]] | ||
| - | [[Category: Blundell, T | + | [[Category: Blundell, T L.]] |
| - | [[Category: Bolanos-Garcia, V | + | [[Category: Bolanos-Garcia, V M.]] |
[[Category: Dhanaraj, V.]] | [[Category: Dhanaraj, V.]] | ||
[[Category: Parisini, E.]] | [[Category: Parisini, E.]] | ||
| - | [[Category: Pei, X | + | [[Category: Pei, X Y.]] |
| - | [[Category: Sayed, M | + | [[Category: Sayed, M F.]] |
[[Category: UNK]] | [[Category: UNK]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zn finger]] | [[Category: zn finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:53:34 2008'' |
Revision as of 12:53, 21 February 2008
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Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide
Overview
A truncated form of the regulatory subunit of the protein kinase CK2beta (residues 1-178) has been crystallized in the presence of a fragment of the cyclin-dependent kinase inhibitor p21WAF1 (residues 46-65) and the structure solved at 2.9 A resolution by molecular replacement. The core of the CK2beta dimer shows a high structural similarity with that identified in previous structural analyses of the dimer and the holoenzyme. However, the electron density corresponding to the substrate-binding acidic loop (residues 55-64) indicates two conformations that differ from that of the holoenzyme structure [Niefind et al. (2001), EMBO J. 20, 5320-5331]. Difference electron density near the dimerization region in each of the eight protomers in the asymmetric unit is attributed to between one and eight amino-acid residues of a complexed fragment of p21WAF1. This binding site corresponds to the solvent-accessible part of the conserved zinc-finger motif.
About this Structure
1RQF is a Single protein structure of sequence from Xenopus laevis with and as ligands. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
Structure of the regulatory subunit of CK2 in the presence of a p21WAF1 peptide demonstrates flexibility of the acidic loop., Bertrand L, Sayed MF, Pei XY, Parisini E, Dhanaraj V, Bolanos-Garcia VM, Allende JE, Blundell TL, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1698-704. Epub, 2004 Sep 23. PMID:15388915
Page seeded by OCA on Thu Feb 21 14:53:34 2008
Categories: Non-specific serine/threonine protein kinase | Single protein | Xenopus laevis | Allende, J E. | Bertrand, L. | Blundell, T L. | Bolanos-Garcia, V M. | Dhanaraj, V. | Parisini, E. | Pei, X Y. | Sayed, M F. | UNK | ZN | Casein kinase beta subunit | Cyclin-dependent kinase inhibitor | Ser/thr protein kinase | Zn finger
