5hpg

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(New page: 200px<br /> <applet load="5hpg" size="450" color="white" frame="true" align="right" spinBox="true" caption="5hpg, resolution 1.66&Aring;" /> '''STRUCTURE AND LIGAN...)
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==About this Structure==
==About this Structure==
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5HPG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5HPG OCA]].
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5HPG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/Plasmin Plasmin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7]]. Structure known Active Site: LBS. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5HPG OCA]].
==Reference==
==Reference==
Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen., Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ, Biochemistry. 1998 Mar 10;37(10):3258-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9521645 9521645]
Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen., Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ, Biochemistry. 1998 Mar 10;37(10):3258-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9521645 9521645]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Plasmin]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Castellino, F.J.]]
[[Category: Castellino, F.J.]]
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[[Category: serine protease]]
[[Category: serine protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:59:02 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:43:11 2007''

Revision as of 11:38, 30 October 2007


5hpg, resolution 1.66Å

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STRUCTURE AND LIGAND DETERMINANTS OF THE RECOMBINANT KRINGLE 5 DOMAIN OF HUMAN PLASMINOGEN

Overview

The X-ray crystal structure of the recombinant (r) kringle 5 domain of, human plasminogen (K5HPg) has been solved by molecular replacement methods, using K1HPg as a model and refined at 1.7 A resolution to an R factor of, 16.6%. The asymmetric unit of K5HPg is composed of two molecules related, by a noncrystallographic 2-fold rotation axis approximately parallel to, the z-direction. The lysine binding site (LBS) is defined by the regions, His33-Thr37, Pro54-Val58, Pro61-Tyr64, and Leu71-Tyr74 and is occupied in, the apo-form by water molecules. A unique feature of the LBS of apo-K5HPg, is the substitution by Leu71 for the basic amino acid, arginine, that in, other kringle polypeptides forms the donor cationic center for the, carboxylate group of omega-amino acid ligands. While wild-type ... [(full description)]

About this Structure

5HPG is a [Single protein] structure of sequence from [Homo sapiens]. Active as [Plasmin], with EC number [3.4.21.7]. Structure known Active Site: LBS. Full crystallographic information is available from [OCA].

Reference

Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen., Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ, Biochemistry. 1998 Mar 10;37(10):3258-71. PMID:9521645

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