1rwd
From Proteopedia
(New page: 200px<br /><applet load="1rwd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rwd" /> '''Backbone NMR Structure of a Mutant P. Furios...) |
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| - | [[Image:1rwd.gif|left|200px]]<br /><applet load="1rwd" size=" | + | [[Image:1rwd.gif|left|200px]]<br /><applet load="1rwd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rwd" /> | caption="1rwd" /> | ||
'''Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings'''<br /> | '''Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A new approach for simultaneous protein backbone resonance assignment and | + | A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed. |
==About this Structure== | ==About this Structure== | ||
| - | 1RWD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure | + | 1RWD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry 1M2Y. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Prestegard, J | + | [[Category: Prestegard, J H.]] |
| - | [[Category: SECSG, Southeast | + | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] |
[[Category: Tian, F.]] | [[Category: Tian, F.]] | ||
[[Category: Valafar, H.]] | [[Category: Valafar, H.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:22 2008'' |
Revision as of 12:55, 21 February 2008
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Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings
Overview
A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed.
About this Structure
1RWD is a Single protein structure of sequence from Pyrococcus furiosus. This structure supersedes the now removed PDB entry 1M2Y. Full crystallographic information is available from OCA.
Reference
A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones., Tian F, Valafar H, Prestegard JH, J Am Chem Soc. 2001 Nov 28;123(47):11791-6. PMID:11716736
Page seeded by OCA on Thu Feb 21 14:55:22 2008
Categories: Pyrococcus furiosus | Single protein | Prestegard, J H. | SECSG, Southeast Collaboratory for Structural Genomics. | Tian, F. | Valafar, H. | Nmr | Protein structure initiative | Psi | Residual dipolar couplings | Secsg | Southeast collaboratory for structural genomics | Structural genomics
