1rxg
From Proteopedia
(New page: 200px<br /><applet load="1rxg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rxg, resolution 1.5Å" /> '''DEACETOXYCEPHALOSPORI...) |
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| - | [[Image:1rxg.jpg|left|200px]]<br /><applet load="1rxg" size=" | + | [[Image:1rxg.jpg|left|200px]]<br /><applet load="1rxg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rxg, resolution 1.5Å" /> | caption="1rxg, resolution 1.5Å" /> | ||
'''DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH FE(II) AND 2-OXOGLUTARATE'''<br /> | '''DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH FE(II) AND 2-OXOGLUTARATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Penicillins and cephalosporins are among the most widely used therapeutic | + | Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation. |
==About this Structure== | ==About this Structure== | ||
| - | 1RXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with FE, SO4 and AKG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1RXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AKG:'>AKG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Streptomyces clavuligerus]] | [[Category: Streptomyces clavuligerus]] | ||
[[Category: Andersson, I.]] | [[Category: Andersson, I.]] | ||
| - | [[Category: Baldwin, J | + | [[Category: Baldwin, J E.]] |
[[Category: Hajdu, J.]] | [[Category: Hajdu, J.]] | ||
[[Category: Hara, T.]] | [[Category: Hara, T.]] | ||
| - | [[Category: Lee, H | + | [[Category: Lee, H J.]] |
| - | [[Category: Lloyd, M | + | [[Category: Lloyd, M D.]] |
[[Category: Perrakis, A.]] | [[Category: Perrakis, A.]] | ||
[[Category: Ramaswamy, S.]] | [[Category: Ramaswamy, S.]] | ||
| - | [[Category: Scheltinga, A | + | [[Category: Scheltinga, A C.Terwisscha Van.]] |
| - | [[Category: Shofield, C | + | [[Category: Shofield, C J.]] |
[[Category: Thompson, A.]] | [[Category: Thompson, A.]] | ||
[[Category: Valegard, K.]] | [[Category: Valegard, K.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:35 2008'' |
Revision as of 12:55, 21 February 2008
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DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH FE(II) AND 2-OXOGLUTARATE
Overview
Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation.
About this Structure
1RXG is a Single protein structure of sequence from Streptomyces clavuligerus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a cephalosporin synthase., Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I, Nature. 1998 Aug 20;394(6695):805-9. PMID:9723623
Page seeded by OCA on Thu Feb 21 14:55:35 2008
Categories: Single protein | Streptomyces clavuligerus | Andersson, I. | Baldwin, J E. | Hajdu, J. | Hara, T. | Lee, H J. | Lloyd, M D. | Perrakis, A. | Ramaswamy, S. | Scheltinga, A C.Terwisscha Van. | Shofield, C J. | Thompson, A. | Valegard, K. | AKG | FE | SO4 | 2-oxoglutarate | Antibiotics | Cephalosporin | Ferrous oxygenase | Merohedral twinning | Oxidoreductase
