1rxi
From Proteopedia
(New page: 200px<br /><applet load="1rxi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rxi, resolution 1.5Å" /> '''pI258 arsenate reduct...) |
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| - | [[Image:1rxi.jpg|left|200px]]<br /><applet load="1rxi" size=" | + | [[Image:1rxi.jpg|left|200px]]<br /><applet load="1rxi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rxi, resolution 1.5Å" /> | caption="1rxi, resolution 1.5Å" /> | ||
'''pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S'''<br /> | '''pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structural insights into formation of the complex between the ubiquitous | + | Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary. |
==About this Structure== | ==About this Structure== | ||
| - | 1RXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with K, LCP and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http:// | + | 1RXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=LCP:'>LCP</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Belle, K | + | [[Category: Belle, K Van.]] |
[[Category: Limbourg, M.]] | [[Category: Limbourg, M.]] | ||
[[Category: Loris, R.]] | [[Category: Loris, R.]] | ||
| - | [[Category: Martins, J | + | [[Category: Martins, J C.]] |
[[Category: Messens, J.]] | [[Category: Messens, J.]] | ||
| - | [[Category: Molle, I | + | [[Category: Molle, I Van.]] |
[[Category: Vanhaesebrouck, P.]] | [[Category: Vanhaesebrouck, P.]] | ||
[[Category: Wahni, K.]] | [[Category: Wahni, K.]] | ||
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[[Category: arsc]] | [[Category: arsc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:45 2008'' |
Revision as of 12:55, 21 February 2008
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pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S
Overview
Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
About this Structure
1RXI is a Single protein structure of sequence from Staphylococcus aureus with , and as ligands. Active as Arsenate reductase (glutaredoxin), with EC number 1.20.4.1 Full crystallographic information is available from OCA.
Reference
The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct., Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1180-4. Epub 2004, May 21. PMID:15159594
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