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1s7z
From Proteopedia
(New page: 200px<br /><applet load="1s7z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s7z, resolution 1.83Å" /> '''Structure of Ocr fro...) |
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| - | [[Image:1s7z.jpg|left|200px]]<br /><applet load="1s7z" size=" | + | [[Image:1s7z.jpg|left|200px]]<br /><applet load="1s7z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s7z, resolution 1.83Å" /> | caption="1s7z, resolution 1.83Å" /> | ||
'''Structure of Ocr from Bacteriophage T7'''<br /> | '''Structure of Ocr from Bacteriophage T7'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have solved, by X-ray crystallography to a resolution of 1.8 A, the | + | We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1S7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1S7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with <scene name='pdbligand=CS:'>CS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Atanasiu, C.]] | [[Category: Atanasiu, C.]] | ||
[[Category: Berg, T.]] | [[Category: Berg, T.]] | ||
| - | [[Category: Dryden, D | + | [[Category: Dryden, D T.]] |
| - | [[Category: Edwardson, J | + | [[Category: Edwardson, J M.]] |
| - | [[Category: Henderson, R | + | [[Category: Henderson, R M.]] |
| - | [[Category: Sturrock, S | + | [[Category: Sturrock, S S.]] |
[[Category: Taylor, P.]] | [[Category: Taylor, P.]] | ||
| - | [[Category: Walkinshaw, M | + | [[Category: Walkinshaw, M D.]] |
[[Category: CS]] | [[Category: CS]] | ||
[[Category: all helical]] | [[Category: all helical]] | ||
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:58:55 2008'' |
Revision as of 12:59, 21 February 2008
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Structure of Ocr from Bacteriophage T7
Overview
We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.
About this Structure
1S7Z is a Single protein structure of sequence from Bacteriophage t7 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597
Page seeded by OCA on Thu Feb 21 14:58:55 2008
