1s8j
From Proteopedia
(New page: 200px<br /><applet load="1s8j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s8j, resolution 2.30Å" /> '''Nitrate-bound D85S m...) |
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| - | [[Image:1s8j.jpg|left|200px]]<br /><applet load="1s8j" size=" | + | [[Image:1s8j.jpg|left|200px]]<br /><applet load="1s8j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s8j, resolution 2.30Å" /> | caption="1s8j, resolution 2.30Å" /> | ||
'''Nitrate-bound D85S mutant of bacteriorhodopsin'''<br /> | '''Nitrate-bound D85S mutant of bacteriorhodopsin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the D85S mutant of bacteriorhodopsin with a nitrate anion | + | The structure of the D85S mutant of bacteriorhodopsin with a nitrate anion bound in the Schiff base binding site and the structure of the anion-free protein have been obtained in the same crystal form. Together with the previously solved structures of this anion pump, in both the anion-free state and bromide-bound state, these new structures provide insight into how this mutant of bacteriorhodopsin is able to bind a variety of different anions in the same binding pocket. The structural analysis reveals that the main structural change that accommodates different anions is the repositioning of the polar side chain of S85. On the basis of these X-ray crystal structures, the prediction is then made that the D85S/D212N double mutant might bind similar anions and do so over a broader pH range than does the single mutant. Experimental comparison of the dissociation constants, K(d), for a variety of anions confirms this prediction and demonstrates, in addition, that the binding affinity is dramatically improved by the D212N substitution. |
==About this Structure== | ==About this Structure== | ||
| - | 1S8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_sp. Halobacterium sp.] with NO3, RET and LI1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1S8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_sp. Halobacterium sp.] with <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=RET:'>RET</scene> and <scene name='pdbligand=LI1:'>LI1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Halobacterium sp.]] | [[Category: Halobacterium sp.]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baliga, N | + | [[Category: Baliga, N S.]] |
| - | [[Category: Cheung, V | + | [[Category: Cheung, V S.]] |
| - | [[Category: Facciotti, M | + | [[Category: Facciotti, M T.]] |
| - | [[Category: Glaeser, R | + | [[Category: Glaeser, R M.]] |
| - | [[Category: Lunde, C | + | [[Category: Lunde, C S.]] |
[[Category: Rouhani, S.]] | [[Category: Rouhani, S.]] | ||
[[Category: LI1]] | [[Category: LI1]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:04 2008'' |
Revision as of 12:59, 21 February 2008
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Nitrate-bound D85S mutant of bacteriorhodopsin
Overview
The structure of the D85S mutant of bacteriorhodopsin with a nitrate anion bound in the Schiff base binding site and the structure of the anion-free protein have been obtained in the same crystal form. Together with the previously solved structures of this anion pump, in both the anion-free state and bromide-bound state, these new structures provide insight into how this mutant of bacteriorhodopsin is able to bind a variety of different anions in the same binding pocket. The structural analysis reveals that the main structural change that accommodates different anions is the repositioning of the polar side chain of S85. On the basis of these X-ray crystal structures, the prediction is then made that the D85S/D212N double mutant might bind similar anions and do so over a broader pH range than does the single mutant. Experimental comparison of the dissociation constants, K(d), for a variety of anions confirms this prediction and demonstrates, in addition, that the binding affinity is dramatically improved by the D212N substitution.
About this Structure
1S8J is a Single protein structure of sequence from Halobacterium sp. with , and as ligands. Full crystallographic information is available from OCA.
Reference
Specificity of anion binding in the substrate pocket of bacteriorhodopsin., Facciotti MT, Cheung VS, Lunde CS, Rouhani S, Baliga NS, Glaeser RM, Biochemistry. 2004 May 4;43(17):4934-43. PMID:15109251
Page seeded by OCA on Thu Feb 21 14:59:04 2008
Categories: Halobacterium sp. | Single protein | Baliga, N S. | Cheung, V S. | Facciotti, M T. | Glaeser, R M. | Lunde, C S. | Rouhani, S. | LI1 | NO3 | RET | Anion | Bacteriorhodopsin | Pump | X-ray diffraction
