1sa3
From Proteopedia
(New page: 200px<br /><applet load="1sa3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sa3, resolution 1.95Å" /> '''An asymmetric comple...) |
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| - | [[Image:1sa3.gif|left|200px]]<br /><applet load="1sa3" size=" | + | [[Image:1sa3.gif|left|200px]]<br /><applet load="1sa3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sa3, resolution 1.95Å" /> | caption="1sa3, resolution 1.95Å" /> | ||
'''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''<br /> | '''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Most well-known restriction endonucleases recognize palindromic DNA | + | Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer. |
==About this Structure== | ==About this Structure== | ||
| - | 1SA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | + | 1SA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
| - | [[Category: Guo, H | + | [[Category: Guo, H C.]] |
| - | [[Category: Kucera, R | + | [[Category: Kucera, R B.]] |
| - | [[Category: Roberts, R | + | [[Category: Roberts, R J.]] |
| - | [[Category: Xu, Q | + | [[Category: Xu, Q S.]] |
[[Category: NA]] | [[Category: NA]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:25 2008'' |
Revision as of 12:59, 21 February 2008
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An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site
Overview
Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
About this Structure
1SA3 is a Single protein structure of sequence from Moraxella sp. with as ligand. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:15341737
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