3rsp
From Proteopedia
(New page: 200px<br /> <applet load="3rsp" size="450" color="white" frame="true" align="right" spinBox="true" caption="3rsp, resolution 1.7Å" /> '''STRUCTURE OF THE P93...) |
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==About this Structure== | ==About this Structure== | ||
| - | 3RSP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3RSP OCA]]. | + | 3RSP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3RSP OCA]]. |
==Reference== | ==Reference== | ||
Structure and stability of the P93G variant of ribonuclease A., Schultz LW, Hargraves SR, Klink TA, Raines RT, Protein Sci. 1998 Jul;7(7):1620-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9684895 9684895] | Structure and stability of the P93G variant of ribonuclease A., Schultz LW, Hargraves SR, Klink TA, Raines RT, Protein Sci. 1998 Jul;7(7):1620-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9684895 9684895] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hargraves, S.R.]] | [[Category: Hargraves, S.R.]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:45:09 2007'' |
Revision as of 11:40, 30 October 2007
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STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A
Overview
The peptide bonds preceding Pro 93 and Pro 114 of bovine pancreatic, ribonuclease A (RNase A) are in the cis conformation. The trans-to-cis, isomerization of these bonds had been indicted as the slow step during, protein folding. Here, site-directed mutagenesis was used to replace Pro, 93 or Pro 114 with a glycine residue, and the crystalline structure of the, P93G variant was determined by X-ray diffraction analysis to a resolution, of 1.7 A. This structure is essentially identical to that of the wild-type, protein, except for the 91-94 beta-turn containing the substitution. In, the wild-type protein, the beta-turn is of type VIa. In the P93G variant, this turn is of type II with the peptide bond preceding Gly 93 being, trans. The thermal stabilities of the P93G and P114G variants were, ... [(full description)]
About this Structure
3RSP is a [Single protein] structure of sequence from [Bos taurus] with CL as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Sites: B1, B2, P1 and P2. Full crystallographic information is available from [OCA].
Reference
Structure and stability of the P93G variant of ribonuclease A., Schultz LW, Hargraves SR, Klink TA, Raines RT, Protein Sci. 1998 Jul;7(7):1620-5. PMID:9684895
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