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1sds
From Proteopedia
(New page: 200px<br /><applet load="1sds" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sds, resolution 1.80Å" /> '''Structure of protein...) |
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| - | [[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size=" | + | [[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sds, resolution 1.80Å" /> | caption="1sds, resolution 1.80Å" /> | ||
'''Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA'''<br /> | '''Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 | + | The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 kDa/Snu13 recognize K-turns. This structural motif is canonically comprised of two stems (one with tandem A.G base pairs, the other with Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes conventional K-turns in ribosomal and box C/D RNAs but also binds specifically to some box H/ACA RNAs at terminal stem loops. These have the A.G paired stem, but lack the Watson-Crick stem. The structure of Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without Watson-Crick stems demonstrates how a binding site for this component of diverse ribonucleoprotein complexes can be constructed with only the A.G stem and the loop. The RNA adopts a functional conformation with the aid of a base triple and tight binding of divalent cations. Comparison with the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic homolog does not recognize terminal stem loop L7Ae binding sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with CA and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A Ferre-D.]] |
[[Category: Hamma, T.]] | [[Category: Hamma, T.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:28 2008'' |
Revision as of 13:00, 21 February 2008
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Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA
Overview
The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 kDa/Snu13 recognize K-turns. This structural motif is canonically comprised of two stems (one with tandem A.G base pairs, the other with Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes conventional K-turns in ribosomal and box C/D RNAs but also binds specifically to some box H/ACA RNAs at terminal stem loops. These have the A.G paired stem, but lack the Watson-Crick stem. The structure of Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without Watson-Crick stems demonstrates how a binding site for this component of diverse ribonucleoprotein complexes can be constructed with only the A.G stem and the loop. The RNA adopts a functional conformation with the aid of a base triple and tight binding of divalent cations. Comparison with the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic homolog does not recognize terminal stem loop L7Ae binding sites.
About this Structure
1SDS is a Single protein structure of sequence from Methanocaldococcus jannaschii with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA at 1.8 A resolution., Hamma T, Ferre-D'Amare AR, Structure. 2004 May;12(5):893-903. PMID:15130481
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