1skr
From Proteopedia
(New page: 200px<br /><applet load="1skr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1skr, resolution 2.4Å" /> '''T7 DNA Polymerase Com...) |
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| - | [[Image:1skr.gif|left|200px]]<br /><applet load="1skr" size=" | + | [[Image:1skr.gif|left|200px]]<br /><applet load="1skr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1skr, resolution 2.4Å" /> | caption="1skr, resolution 2.4Å" /> | ||
'''T7 DNA Polymerase Complexed To DNA Primer/Template and ddATP'''<br /> | '''T7 DNA Polymerase Complexed To DNA Primer/Template and ddATP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ultraviolet-induced DNA damage poses a lethal block to replication. To | + | Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion. |
==About this Structure== | ==About this Structure== | ||
| - | 1SKR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and DAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1SKR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=DAD:'>DAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bdour, H | + | [[Category: Bdour, H M.]] |
[[Category: Doublie, S.]] | [[Category: Doublie, S.]] | ||
[[Category: Dutta, S.]] | [[Category: Dutta, S.]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
[[Category: Li, Y.]] | [[Category: Li, Y.]] | ||
| - | [[Category: Taylor, J | + | [[Category: Taylor, J S.]] |
[[Category: DAD]] | [[Category: DAD]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: uv-lesion]] | [[Category: uv-lesion]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:28 2008'' |
Revision as of 13:02, 21 February 2008
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T7 DNA Polymerase Complexed To DNA Primer/Template and ddATP
Overview
Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.
About this Structure
1SKR is a Protein complex structure of sequences from Bacteriophage t7 and Escherichia coli with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7., Li Y, Dutta S, Doublie S, Bdour HM, Taylor JS, Ellenberger T, Nat Struct Mol Biol. 2004 Aug;11(8):784-90. Epub 2004 Jul 4. PMID:15235589
Page seeded by OCA on Thu Feb 21 15:02:28 2008
Categories: Bacteriophage t7 | DNA-directed DNA polymerase | Escherichia coli | Protein complex | Bdour, H M. | Doublie, S. | Dutta, S. | Ellenberger, T. | Li, Y. | Taylor, J S. | DAD | MG | Close | Dna polymerase | Open | Replication | Uv-lesion
