1smp
From Proteopedia
(New page: 200px<br /><applet load="1smp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1smp, resolution 2.30Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1smp.jpg|left|200px]]<br /><applet load="1smp" size=" | + | [[Image:1smp.jpg|left|200px]]<br /><applet load="1smp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1smp, resolution 2.30Å" /> | caption="1smp, resolution 2.30Å" /> | ||
'''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI'''<br /> | '''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the complex between the 50 kDa | + | The crystal structure of the complex between the 50 kDa metallo-endoproteinase from Serratia marcescens (SMP), a member of the metzincin superfamily, and an inhibitor from Erwinia chrysanthemi (Inh) was solved by molecular replacement using the known structure of SMP, and refined at 2.30 A resolution to a crystallographic R-factor of 0.195. The E. chrysanthemi inhibitor folds into a compact eight-stranded antiparallel beta-barrel of simple up-down topology such as is found for members of the retinol binding protein family. It mainly interacts with the protease via its five N-terminal residues, which insert into the active site cleft, occupying the S' sites. The first N-terminal residue, SerI1, is partially cleaved off by the protease, while SerI2 makes a hydrogen bond with the catalytically active glutamic acid, Glu177, of the protease. Further interactions are made between one face of the inhibitor formed by the strands s3, s4 and s5 and the protease segment 218 to 228, which is located immediately after the characteristic "Met-turn" of the metzincins. |
==About this Structure== | ==About this Structure== | ||
| - | 1SMP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] and [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Serralysin Serralysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.40 3.4.24.40] Full crystallographic information is available from [http:// | + | 1SMP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] and [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Serralysin Serralysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.40 3.4.24.40] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: complex (metalloprotease/inhibitor)]] | [[Category: complex (metalloprotease/inhibitor)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:03:06 2008'' |
Revision as of 13:03, 21 February 2008
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CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI
Overview
The crystal structure of the complex between the 50 kDa metallo-endoproteinase from Serratia marcescens (SMP), a member of the metzincin superfamily, and an inhibitor from Erwinia chrysanthemi (Inh) was solved by molecular replacement using the known structure of SMP, and refined at 2.30 A resolution to a crystallographic R-factor of 0.195. The E. chrysanthemi inhibitor folds into a compact eight-stranded antiparallel beta-barrel of simple up-down topology such as is found for members of the retinol binding protein family. It mainly interacts with the protease via its five N-terminal residues, which insert into the active site cleft, occupying the S' sites. The first N-terminal residue, SerI1, is partially cleaved off by the protease, while SerI2 makes a hydrogen bond with the catalytically active glutamic acid, Glu177, of the protease. Further interactions are made between one face of the inhibitor formed by the strands s3, s4 and s5 and the protease segment 218 to 228, which is located immediately after the characteristic "Met-turn" of the metzincins.
About this Structure
1SMP is a Protein complex structure of sequences from Erwinia chrysanthemi and Serratia marcescens with and as ligands. Active as Serralysin, with EC number 3.4.24.40 Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Serratia marcescens metallo-protease and an inhibitor from Erwinia chrysanthemi., Baumann U, Bauer M, Letoffe S, Delepelaire P, Wandersman C, J Mol Biol. 1995 May 5;248(3):653-61. PMID:7752231
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