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1he8
From Proteopedia
(New page: 200px<br /> <applet load="1he8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1he8, resolution 3.00Å" /> '''RAS G12V-PI 3-KINAS...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HE8 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and GNP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.137 2.7.1.137]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HE8 OCA]]. | + | 1HE8 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and GNP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phosphatidylinositol_3-kinase Phosphatidylinositol 3-kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.137 2.7.1.137]]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HE8 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma., Pacold ME, Suire S, Perisic O, Lara-Gonzalez S, Davis CT, Walker EH, Hawkins PT, Stephens L, Eccleston JF, Williams RL, Cell. 2000 Dec 8;103(6):931-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11136978 11136978] | Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma., Pacold ME, Suire S, Perisic O, Lara-Gonzalez S, Davis CT, Walker EH, Hawkins PT, Stephens L, Eccleston JF, Williams RL, Cell. 2000 Dec 8;103(6):931-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11136978 11136978] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| + | [[Category: Phosphatidylinositol 3-kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Davis, C.T.]] | [[Category: Davis, C.T.]] | ||
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[[Category: second messenger generation]] | [[Category: second messenger generation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:47:22 2007'' |
Revision as of 11:42, 30 October 2007
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RAS G12V-PI 3-KINASE GAMMA COMPLEX
Overview
Ras activation of phosphoinositide 3-kinase (PI3K) is important for, survival of transformed cells. We find that PI3Kgamma is strongly and, directly activated by H-Ras G12V in vivo or by GTPgammaS-loaded H-Ras in, vitro. We have determined a crystal structure of a PI3Kgamma/Ras.GMPPNP, complex. A critical loop in the Ras binding domain positions Ras so that, it uses its switch I and switch II regions to bind PI3Kgamma. Mutagenesis, shows that interactions with both regions are essential for binding, PI3Kgamma. Ras also forms a direct contact with the PI3Kgamma catalytic, domain. These unique Ras/PI3Kgamma interactions are likely to be shared by, PI3Kalpha. The complex with Ras shows a change in the PI3K conformation, that may represent an allosteric component of Ras activation.
About this Structure
1HE8 is a [Protein complex] structure of sequences from [Homo sapiens] with MG and GNP as [ligands]. Active as [Phosphatidylinositol 3-kinase], with EC number [2.7.1.137]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [OCA].
Reference
Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma., Pacold ME, Suire S, Perisic O, Lara-Gonzalez S, Davis CT, Walker EH, Hawkins PT, Stephens L, Eccleston JF, Williams RL, Cell. 2000 Dec 8;103(6):931-43. PMID:11136978
Page seeded by OCA on Tue Oct 30 13:47:22 2007
Categories: Homo sapiens | Phosphatidylinositol 3-kinase | Protein complex | Davis, C.T. | Eccleston, J.F. | Hawkins, P.T. | Lara-Gonzalez, S. | Pacold, M.E. | Perisic, O. | Stephens, L. | Suire, S. | Walker, E.H. | Williams, R.L. | GNP | MG | Gmppnp | Gtp | H-ras g12v | Heat domain | Oncogene protein | Phosphoinositide 3-kinase gamma - h-ras g12v complex | Pi 3-k | Pi 3-kinase | Pi3k | Ras effector | Ras-binding domain | Second messenger generation
