1gim
From Proteopedia
(New page: 200px<br /> <applet load="1gim" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gim, resolution 2.5Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GIM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, NO3, HAD, IMP and GDP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GIM OCA]]. | + | 1GIM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, NO3, HAD, IMP and GDP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]]. Structure known Active Sites: ASP, GNS and IMP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GIM OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+., Poland BW, Fromm HJ, Honzatko RB, J Mol Biol. 1996 Dec 20;264(5):1013-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9000627 9000627] | Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+., Poland BW, Fromm HJ, Honzatko RB, J Mol Biol. 1996 Dec 20;264(5):1013-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9000627 9000627] | ||
| + | [[Category: Adenylosuccinate synthase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:48:52 2007'' |
Revision as of 11:44, 30 October 2007
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CRYSTAL STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH GDP, IMP, HADACIDIN, NO3-, AND MG2+. DATA COLLECTED AT 100K (PH 6.5)
Overview
Crystal structures of adenylosuccinate synthetase from Esherichia coli, complexed with Mg2+, IMP, GDP, NO3- and hadacidin at 298 and 100 K have, been refined to R-factors of 0.188 and 0.206 against data to 2.8 A and 2.5, A resolution, respectively. Conformational changes of up to 9 A relative, to the unligated enzyme occur in loops that bind to Mg2+, GDP, IMP and, hadacidin. Mg2+ binds directly to GDP, NO3-, hadacidin and the protein, but is only five-coordinated. Asp13, which approaches, but does not occupy, the sixth coordination site of Mg2+, hydrogen bonds to N1 of IMP. The, nitrogen atom of NO3- is approximately 2.7 A from O6 of IMP, reflecting a, strong electrostatic interaction between the electron-deficient nitrogen, atom and the electron-rich O6. The spatial relationships between ... [(full description)]
About this Structure
1GIM is a [Single protein] structure of sequence from [Escherichia coli] with MG, NO3, HAD, IMP and GDP as [ligands]. Active as [Adenylosuccinate synthase], with EC number [6.3.4.4]. Structure known Active Sites: ASP, GNS and IMP. Full crystallographic information is available from [OCA].
Reference
Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+., Poland BW, Fromm HJ, Honzatko RB, J Mol Biol. 1996 Dec 20;264(5):1013-27. PMID:9000627
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