1t5t
From Proteopedia
(New page: 200px<br /><applet load="1t5t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t5t, resolution 2.90Å" /> '''Structure of the (SR...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1t5t.gif|left|200px]]<br /><applet load="1t5t" size=" | + | [[Image:1t5t.gif|left|200px]]<br /><applet load="1t5t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t5t, resolution 2.90Å" /> | caption="1t5t, resolution 2.90Å" /> | ||
'''Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form'''<br /> | '''Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A tight coupling between adenosine triphosphate (ATP) hydrolysis and | + | A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane. |
==About this Structure== | ==About this Structure== | ||
| - | 1T5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with ALF, CA, MG, K and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http:// | + | 1T5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=ALF:'>ALF</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5T OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Moller, J | + | [[Category: Moller, J V.]] |
[[Category: Nissen, P.]] | [[Category: Nissen, P.]] | ||
| - | [[Category: Sorensen, T | + | [[Category: Sorensen, T L.M.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: ALF]] | [[Category: ALF]] | ||
| Line 29: | Line 29: | ||
[[Category: transition state]] | [[Category: transition state]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:12 2008'' |
Revision as of 13:10, 21 February 2008
|
Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form
Overview
A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane.
About this Structure
1T5T is a Single protein structure of sequence from Oryctolagus cuniculus with , , , and as ligands. Active as Calcium-transporting ATPase, with EC number 3.6.3.8 Full crystallographic information is available from OCA.
Reference
Phosphoryl transfer and calcium ion occlusion in the calcium pump., Sorensen TL, Moller JV, Nissen P, Science. 2004 Jun 11;304(5677):1672-5. PMID:15192230
Page seeded by OCA on Thu Feb 21 15:10:12 2008
