1w6y
From Proteopedia
(New page: 200px<br /> <applet load="1w6y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w6y, resolution 2.1Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1W6Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with EQU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W6Y OCA]]. | + | 1W6Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with EQU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W6Y OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Steroid Delta-isomerase]] | ||
[[Category: Choi, K.Y.]] | [[Category: Choi, K.Y.]] | ||
[[Category: Kim, Y.G.]] | [[Category: Kim, Y.G.]] | ||
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[[Category: solvent-exposed]] | [[Category: solvent-exposed]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:50:35 2007'' |
Revision as of 11:45, 30 October 2007
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CRYSTAL STRUCTURE OF A MUTANT W92A IN KETOSTEROID ISOMERASE (KSI) FROM PSEUDOMONAS PUTIDA BIOTYPE B
Overview
A structural motif called the small exterior hydrophobic cluster (SEHC), has been proposed to explain the stabilizing effect mediated by, solvent-exposed hydrophobic residues; however, little is known about its, biological roles. Unusually, in Delta(5)-3-ketosteroid isomerase from, Pseudomonas putida biotype B (KSI-PI) Trp92 is exposed to solvent on the, protein surface, forming a SEHC with the side-chains of Leu125 and Val127., In order to identify the role of the SEHC in KSI-PI, mutants of those, amino acids associated with the SEHC were prepared. The W92A, L125A/V127A, and W92A/L125A/V127A mutations largely decreased the conformational, stability, while the L125F/V127F mutation slightly increased the, stability, indicating that hydrophobic packing by the SEHC is important in, ... [(full description)]
About this Structure
1W6Y is a [Single protein] structure of sequence from [Pseudomonas putida] with EQU and BME as [ligands]. Active as [Steroid Delta-isomerase], with EC number [5.3.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B., Yun YS, Nam GH, Kim YG, Oh BH, Choi KY, FEBS J. 2005 Apr;272(8):1999-2011. PMID:15819891
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