1tlw
From Proteopedia
(New page: 200px<br /><applet load="1tlw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tlw, resolution 3.10Å" /> '''Tsx structure comple...) |
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| - | [[Image:1tlw.jpg|left|200px]]<br /><applet load="1tlw" size=" | + | [[Image:1tlw.jpg|left|200px]]<br /><applet load="1tlw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tlw, resolution 3.10Å" /> | caption="1tlw, resolution 3.10Å" /> | ||
'''Tsx structure complexed with thymidine'''<br /> | '''Tsx structure complexed with thymidine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Tsx is a nucleoside-specific outer membrane (OM) transporter of | + | Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next. |
==About this Structure== | ==About this Structure== | ||
| - | 1TLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with THM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=THM:'>THM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berg, B | + | [[Category: Berg, B van den.]] |
[[Category: Ye, J.]] | [[Category: Ye, J.]] | ||
[[Category: THM]] | [[Category: THM]] | ||
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[[Category: thymidine]] | [[Category: thymidine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:57 2008'' |
Revision as of 13:15, 21 February 2008
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Tsx structure complexed with thymidine
Overview
Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.
About this Structure
1TLW is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the bacterial nucleoside transporter Tsx., Ye J, van den Berg B, EMBO J. 2004 Aug 18;23(16):3187-95. Epub 2004 Jul 22. PMID:15272310
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