1tro
From Proteopedia
(New page: 200px<br /><applet load="1tro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tro, resolution 1.900Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1tro.gif|left|200px]]<br /><applet load="1tro" size=" | + | [[Image:1tro.gif|left|200px]]<br /><applet load="1tro" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tro, resolution 1.900Å" /> | caption="1tro, resolution 1.900Å" /> | ||
'''CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the trp repressor/operator complex shows an | + | The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1TRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=TRP:'>TRP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Joachimiak, A | + | [[Category: Joachimiak, A J.]] |
| - | [[Category: Lawson, C | + | [[Category: Lawson, C L.]] |
| - | [[Category: Luisi, B | + | [[Category: Luisi, B F.]] |
[[Category: Marmorstein, R.]] | [[Category: Marmorstein, R.]] | ||
[[Category: Otwinowski, Z.]] | [[Category: Otwinowski, Z.]] | ||
| - | [[Category: Schevitz, R | + | [[Category: Schevitz, R W.]] |
| - | [[Category: Sigler, P | + | [[Category: Sigler, P B.]] |
| - | [[Category: Zhang, R | + | [[Category: Zhang, R G.]] |
[[Category: TRP]] | [[Category: TRP]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:16:39 2008'' |
Revision as of 13:16, 21 February 2008
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CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION
Overview
The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity.
About this Structure
1TRO is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of trp repressor/operator complex at atomic resolution., Otwinowski Z, Schevitz RW, Zhang RG, Lawson CL, Joachimiak A, Marmorstein RQ, Luisi BF, Sigler PB, Nature. 1988 Sep 22;335(6188):321-9. PMID:3419502
Page seeded by OCA on Thu Feb 21 15:16:39 2008
