2blw
From Proteopedia
(New page: 200px<br /> <applet load="2blw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2blw, resolution 1.20Å" /> '''TRYPSIN AFTER A HIG...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BLW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CA, SO4, BEN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLW OCA]]. | + | 2BLW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CA, SO4, BEN and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Trypsin Trypsin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLW OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Trypsin]] | ||
[[Category: Nanao, M.H.]] | [[Category: Nanao, M.H.]] | ||
[[Category: Ravelli, R.B.]] | [[Category: Ravelli, R.B.]] | ||
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[[Category: synchrotron]] | [[Category: synchrotron]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:51:28 2007'' |
Revision as of 11:46, 30 October 2007
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TRYPSIN AFTER A HIGH DOSE X-RAY "BURN"
Overview
Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [(full description)]
About this Structure
2BLW is a [Single protein] structure of sequence from [Bos taurus] with CA, SO4, BEN and GOL as [ligands]. Active as [Trypsin], with EC number [3.4.21.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756
Page seeded by OCA on Tue Oct 30 13:51:28 2007
Categories: Bos taurus | Single protein | Trypsin | Nanao, M.H. | Ravelli, R.B. | BEN | CA | GOL | SO4 | Calcium-binding | Digestion | Hydrolase | Pancreas | Phasing | Protease | Radiation damage | Rip | Serine protease | Synchrotron
