2bli
From Proteopedia
(New page: 200px<br /> <applet load="2bli" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bli, resolution 1.7Å" /> '''L29W MB DEOXY'''<br ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BLI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]] with HEM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLI OCA]]. | + | 2BLI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]] with HEM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLI OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: oxygen transport]] | [[Category: oxygen transport]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:51:48 2007'' |
Revision as of 11:47, 30 October 2007
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L29W MB DEOXY
Overview
We have determined eight X-ray structures of myoglobin mutant L29W at, various experimental conditions. In addition, infrared spectroscopic, experiments are presented, which are discussed in the light of the X-ray, structures. Two distinct conformations of the CO-ligated protein were, identified, giving rise to two stretching bands of heme-bound CO. If L29W, MbCO crystals are illuminated around 180 K, a deoxy species is formed. The, CO molecules migrate to the proximal side of the heme and remain trapped, in the so-called Xe1 cavity upon temperature decrease to 105 K. The, structure of this photoproduct is almost identical to the equilibrium, high-temperature deoxy Mb structure. If the temperature is cycled to, increasingly higher values, CO recombination is observed. Three, intermediate ... [(full description)]
About this Structure
2BLI is a [Single protein] structure of sequence from [Physeter catodon] with HEM as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Ligand migration and protein fluctuations in myoglobin mutant L29W., Nienhaus K, Ostermann A, Nienhaus GU, Parak FG, Schmidt M, Biochemistry. 2005 Apr 5;44(13):5095-105. PMID:15794647
Page seeded by OCA on Tue Oct 30 13:51:48 2007
Categories: Physeter catodon | Single protein | Nienhaus, G.U. | Nienhaus, K. | Ostermann, A. | Parak, F.G. | Schmidt, M. | HEM | Heme | Mutant | Myoglobin | Oxygen transport
