1tzy
From Proteopedia
(New page: 200px<br /><applet load="1tzy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tzy, resolution 1.90Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1tzy.jpg|left|200px]]<br /><applet load="1tzy" size=" | + | [[Image:1tzy.jpg|left|200px]]<br /><applet load="1tzy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tzy, resolution 1.90Å" /> | caption="1tzy, resolution 1.90Å" /> | ||
'''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution'''<br /> | '''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystals of native histone octamers | + | Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle. |
==About this Structure== | ==About this Structure== | ||
| - | 1TZY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with PO4 and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1TZY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZY OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta | + | High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511091 16511091] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Baldwin, J | + | [[Category: Baldwin, J P.]] |
[[Category: Chantalat, L.]] | [[Category: Chantalat, L.]] | ||
| - | [[Category: Lambert, S | + | [[Category: Lambert, S J.]] |
| - | [[Category: Nicholson, J | + | [[Category: Nicholson, J M.]] |
| - | [[Category: Reynolds, C | + | [[Category: Reynolds, C D.]] |
| - | [[Category: Wood, C | + | [[Category: Wood, C M.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| Line 24: | Line 24: | ||
[[Category: tetramer-dimer-dimer]] | [[Category: tetramer-dimer-dimer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:11 2008'' |
Revision as of 13:19, 21 February 2008
|
Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution
Overview
Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle.
About this Structure
1TZY is a Protein complex structure of sequences from Gallus gallus with and as ligands. Full crystallographic information is available from OCA.
Reference
High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:16511091
Page seeded by OCA on Thu Feb 21 15:19:11 2008
