1u19
From Proteopedia
(New page: 200px<br /><applet load="1u19" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u19, resolution 2.2Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1u19.gif|left|200px]]<br /><applet load="1u19" size=" | + | [[Image:1u19.gif|left|200px]]<br /><applet load="1u19" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u19, resolution 2.2Å" /> | caption="1u19, resolution 2.2Å" /> | ||
'''Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution'''<br /> | '''Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A new high-resolution structure is reported for bovine rhodopsin, the | + | A new high-resolution structure is reported for bovine rhodopsin, the visual pigment in rod photoreceptor cells. Substantial improvement of the resolution limit to 2.2 A has been achieved by new crystallization conditions, which also reduce significantly the probability of merohedral twinning in the crystals. The new structure completely resolves the polypeptide chain and provides further details of the chromophore binding site including the configuration about the C6-C7 single bond of the 11-cis-retinal Schiff base. Based on both an earlier structure and the new improved model of the protein, a theoretical study of the chromophore geometry has been carried out using combined quantum mechanics/force field molecular dynamics. The consistency between the experimental and calculated chromophore structures is found to be significantly improved for the 2.2 A model, including the angle of the negatively twisted 6-s-cis-bond. Importantly, the new crystal structure refinement reveals significant negative pre-twist of the C11-C12 double bond and this is also supported by the theoretical calculation although the latter converges to a smaller value. Bond alternation along the unsaturated chain is significant, but weaker in the calculated structure than the one obtained from the X-ray data. Other differences between the experimental and theoretical structures in the chromophore binding site are discussed with respect to the unique spectral properties and excited state reactivity of the chromophore. |
==About this Structure== | ==About this Structure== | ||
| - | 1U19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with HG, ZN, ACE, RET, PLM, HTO and HTG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1U19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=ACE:'>ACE</scene>, <scene name='pdbligand=RET:'>RET</scene>, <scene name='pdbligand=PLM:'>PLM</scene>, <scene name='pdbligand=HTO:'>HTO</scene> and <scene name='pdbligand=HTG:'>HTG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U19 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bondar, A | + | [[Category: Bondar, A N.]] |
[[Category: Buss, V.]] | [[Category: Buss, V.]] | ||
[[Category: Elstner, M.]] | [[Category: Elstner, M.]] | ||
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[[Category: retinal protein]] | [[Category: retinal protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:34 2008'' |
Revision as of 13:19, 21 February 2008
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Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution
Overview
A new high-resolution structure is reported for bovine rhodopsin, the visual pigment in rod photoreceptor cells. Substantial improvement of the resolution limit to 2.2 A has been achieved by new crystallization conditions, which also reduce significantly the probability of merohedral twinning in the crystals. The new structure completely resolves the polypeptide chain and provides further details of the chromophore binding site including the configuration about the C6-C7 single bond of the 11-cis-retinal Schiff base. Based on both an earlier structure and the new improved model of the protein, a theoretical study of the chromophore geometry has been carried out using combined quantum mechanics/force field molecular dynamics. The consistency between the experimental and calculated chromophore structures is found to be significantly improved for the 2.2 A model, including the angle of the negatively twisted 6-s-cis-bond. Importantly, the new crystal structure refinement reveals significant negative pre-twist of the C11-C12 double bond and this is also supported by the theoretical calculation although the latter converges to a smaller value. Bond alternation along the unsaturated chain is significant, but weaker in the calculated structure than the one obtained from the X-ray data. Other differences between the experimental and theoretical structures in the chromophore binding site are discussed with respect to the unique spectral properties and excited state reactivity of the chromophore.
About this Structure
1U19 is a Single protein structure of sequence from Bos taurus with , , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure., Okada T, Sugihara M, Bondar AN, Elstner M, Entel P, Buss V, J Mol Biol. 2004 Sep 10;342(2):571-83. PMID:15327956
Page seeded by OCA on Thu Feb 21 15:19:34 2008
Categories: Bos taurus | Single protein | Bondar, A N. | Buss, V. | Elstner, M. | Entel, P. | Okada, T. | Sugihara, M. | ACE | HG | HTG | HTO | PLM | RET | ZN | G protein-coupled receptor | Membrane protein | Photoreceptor | Retinal protein
