1u8b
From Proteopedia
(New page: 200px<br /><applet load="1u8b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u8b, resolution 2.10Å" /> '''Crystal structure of...) |
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| - | [[Image:1u8b.gif|left|200px]]<br /><applet load="1u8b" size=" | + | [[Image:1u8b.gif|left|200px]]<br /><applet load="1u8b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u8b, resolution 2.10Å" /> | caption="1u8b, resolution 2.10Å" /> | ||
'''Crystal structure of the methylated N-ADA/DNA complex'''<br /> | '''Crystal structure of the methylated N-ADA/DNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The transcriptional activity of many sequence-specific DNA binding | + | The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor controlled by posttranslational modification is E. coli Ada protein, a chemosensor that both repairs methylation damage in DNA and coordinates the resistance response to genotoxic methylating agents. Ada repairs methyl phosphotriester lesions in DNA by transferring the aberrant methyl group to one of its own cysteine residues; this site-specific methylation enhances tremendously the DNA binding activity of the protein, thereby enabling it to activate a methylation-resistance regulon. Here, we report solution and X-ray structures of the Cys-methylated chemosensor domain of Ada bound to DNA. The structures reveal that both phosphotriester repair and methylation-dependent transcriptional activation function through a zinc- and methylation-dependent electrostatic switch. |
==About this Structure== | ==About this Structure== | ||
| - | 1U8B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1U8B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dotsch, V.]] | [[Category: Dotsch, V.]] | ||
| - | [[Category: Gross, J | + | [[Category: Gross, J D.]] |
[[Category: He, C.]] | [[Category: He, C.]] | ||
| - | [[Category: Hus, J | + | [[Category: Hus, J C.]] |
| - | [[Category: Lane, W | + | [[Category: Lane, W S.]] |
| - | [[Category: Norman, D | + | [[Category: Norman, D P.G.]] |
| - | [[Category: Sun, L | + | [[Category: Sun, L J.]] |
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: Zhou, P.]] | [[Category: Zhou, P.]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:39 2008'' |
Revision as of 13:21, 21 February 2008
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Crystal structure of the methylated N-ADA/DNA complex
Overview
The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor controlled by posttranslational modification is E. coli Ada protein, a chemosensor that both repairs methylation damage in DNA and coordinates the resistance response to genotoxic methylating agents. Ada repairs methyl phosphotriester lesions in DNA by transferring the aberrant methyl group to one of its own cysteine residues; this site-specific methylation enhances tremendously the DNA binding activity of the protein, thereby enabling it to activate a methylation-resistance regulon. Here, we report solution and X-ray structures of the Cys-methylated chemosensor domain of Ada bound to DNA. The structures reveal that both phosphotriester repair and methylation-dependent transcriptional activation function through a zinc- and methylation-dependent electrostatic switch.
About this Structure
1U8B is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
A methylation-dependent electrostatic switch controls DNA repair and transcriptional activation by E. coli ada., He C, Hus JC, Sun LJ, Zhou P, Norman DP, Dotsch V, Wei H, Gross JD, Lane WS, Wagner G, Verdine GL, Mol Cell. 2005 Oct 7;20(1):117-29. PMID:16209950
Page seeded by OCA on Thu Feb 21 15:21:39 2008
Categories: Escherichia coli | Single protein | Dotsch, V. | Gross, J D. | He, C. | Hus, J C. | Lane, W S. | Norman, D P.G. | Sun, L J. | Verdine, G L. | Wagner, G. | Zhou, P. | ZN | Helix-turn-helix | Methylation | Protein-dna complex | Zinc
