1u90
From Proteopedia
(New page: 200px<br /><applet load="1u90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u90, resolution 2.00Å" /> '''Crystal structures o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1u90.jpg|left|200px]]<br /><applet load="1u90" size=" | + | [[Image:1u90.jpg|left|200px]]<br /><applet load="1u90" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u90, resolution 2.00Å" /> | caption="1u90, resolution 2.00Å" /> | ||
'''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap'''<br /> | '''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst | + | RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap. |
==About this Structure== | ==About this Structure== | ||
| - | 1U90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1U90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U90 OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Kosak, J.]] | [[Category: Kosak, J.]] | ||
[[Category: Mattos, C.]] | [[Category: Mattos, C.]] | ||
| - | [[Category: Nicely, N | + | [[Category: Nicely, N I.]] |
| - | [[Category: Serrano, V | + | [[Category: Serrano, V de.]] |
[[Category: GDP]] | [[Category: GDP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 28: | Line 28: | ||
[[Category: ras]] | [[Category: ras]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:52 2008'' |
Revision as of 13:21, 21 February 2008
|
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U90 is a Single protein structure of sequence from Saguinus oedipus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Thu Feb 21 15:21:52 2008
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N I. | Serrano, V de. | GDP | MG | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ras
