1ual
From Proteopedia
(New page: 200px<br /><applet load="1ual" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ual, resolution 1.80Å" /> '''Crystal structure of...) |
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| - | [[Image:1ual.gif|left|200px]]<br /><applet load="1ual" size=" | + | [[Image:1ual.gif|left|200px]]<br /><applet load="1ual" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ual, resolution 1.80Å" /> | caption="1ual, resolution 1.80Å" /> | ||
'''Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition'''<br /> | '''Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition'''<br /> | ||
==Overview== | ==Overview== | ||
| - | tRNA(m(1)G37)methyltransferase (TrmD) catalyzes the transfer of a methyl | + | tRNA(m(1)G37)methyltransferase (TrmD) catalyzes the transfer of a methyl group from S-adenosyl-L- methionine (AdoMet) to G(37) within a subset of bacterial tRNA species, which have a G residue at the 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. Here we report four crystal structures of TrmD from Haemophilus influenzae, as binary complexes with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy and phosphate, and as an apo form. This first structure of TrmD indicates that it functions as a dimer. It also suggests the binding mode of G(36)G(37) in the active site of TrmD and the catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows structural similarity to trp repressor. We propose a plausible model for the TrmD(2)-tRNA(2) complex, which provides insights into recognition of the general tRNA structure by TrmD. |
==About this Structure== | ==About this Structure== | ||
| - | 1UAL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/tRNA_(guanine-N(1)-)-methyltransferase tRNA (guanine-N(1)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.31 2.1.1.31] Full crystallographic information is available from [http:// | + | 1UAL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/tRNA_(guanine-N(1)-)-methyltransferase tRNA (guanine-N(1)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.31 2.1.1.31] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: tRNA (guanine-N(1)-)-methyltransferase]] | [[Category: tRNA (guanine-N(1)-)-methyltransferase]] | ||
| - | [[Category: Ahn, H | + | [[Category: Ahn, H J.]] |
| - | [[Category: Kim, H | + | [[Category: Kim, H W.]] |
| - | [[Category: Lee, B | + | [[Category: Lee, B I.]] |
| - | [[Category: Suh, S | + | [[Category: Suh, S W.]] |
| - | [[Category: Yang, J | + | [[Category: Yang, J K.]] |
| - | [[Category: Yoon, H | + | [[Category: Yoon, H J.]] |
[[Category: SAH]] | [[Category: SAH]] | ||
[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
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[[Category: trna(m1g37)methyltransferase]] | [[Category: trna(m1g37)methyltransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:21 2008'' |
Revision as of 13:22, 21 February 2008
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Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition
Overview
tRNA(m(1)G37)methyltransferase (TrmD) catalyzes the transfer of a methyl group from S-adenosyl-L- methionine (AdoMet) to G(37) within a subset of bacterial tRNA species, which have a G residue at the 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. Here we report four crystal structures of TrmD from Haemophilus influenzae, as binary complexes with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy and phosphate, and as an apo form. This first structure of TrmD indicates that it functions as a dimer. It also suggests the binding mode of G(36)G(37) in the active site of TrmD and the catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows structural similarity to trp repressor. We propose a plausible model for the TrmD(2)-tRNA(2) complex, which provides insights into recognition of the general tRNA structure by TrmD.
About this Structure
1UAL is a Single protein structure of sequence from Haemophilus influenzae with as ligand. Active as tRNA (guanine-N(1)-)-methyltransferase, with EC number 2.1.1.31 Full crystallographic information is available from OCA.
Reference
Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition., Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK, EMBO J. 2003 Jun 2;22(11):2593-603. PMID:12773376
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