1uco
From Proteopedia
(New page: 200px<br /><applet load="1uco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uco, resolution 2.0Å" /> '''HEN EGG-WHITE LYSOZYM...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1uco.jpg|left|200px]]<br /><applet load="1uco" size=" | + | [[Image:1uco.jpg|left|200px]]<br /><applet load="1uco" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1uco, resolution 2.0Å" /> | caption="1uco, resolution 2.0Å" /> | ||
'''HEN EGG-WHITE LYSOZYME, LOW HUMIDITY FORM'''<br /> | '''HEN EGG-WHITE LYSOZYME, LOW HUMIDITY FORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The atomic models of native monoclinic lysozyme obtained by refinement at | + | The atomic models of native monoclinic lysozyme obtained by refinement at Bangalore and elsewhere [Young, Dewan, Nave & Tilton (1993). J. Appl. Cryst. 26, 309-319] differed significantly in the flexible regions of the protein molecule. The two models were reconciled starting from regions where they were in reasonable agreement to produce an improved model which yielded an R value of 0.169 for 12 816 observed reflections in the 10-2 A resolution range. The reconciled model was compared with the structure of the 88% relative humidity form obtained through a water-mediated transformation [Madhusudan, Kodandapani & Vijayan (1993). Acta Cryst. D49, 234-245]. Parts of the flexible regions of the molecule register significant movements during the transformation. The changes resulting from the transformation from the native to the low-humidity forms are pronounced in many of the side chains in the active-site region, thus indicating the relationship between hydration, mobility and enzyme action. The fact that the overall changes in molecular geometry resulting from water-mediated transformation are similar to those which occur during enzyme action, further emphasizes this relationship. |
==About this Structure== | ==About this Structure== | ||
| - | 1UCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1UCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCO OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Nagendra, H | + | [[Category: Nagendra, H G.]] |
[[Category: Sudarsanakumar, C.]] | [[Category: Sudarsanakumar, C.]] | ||
[[Category: Vijayan, M.]] | [[Category: Vijayan, M.]] | ||
| Line 21: | Line 21: | ||
[[Category: o-glycosyl]] | [[Category: o-glycosyl]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:02 2008'' |
Revision as of 13:23, 21 February 2008
|
HEN EGG-WHITE LYSOZYME, LOW HUMIDITY FORM
Overview
The atomic models of native monoclinic lysozyme obtained by refinement at Bangalore and elsewhere [Young, Dewan, Nave & Tilton (1993). J. Appl. Cryst. 26, 309-319] differed significantly in the flexible regions of the protein molecule. The two models were reconciled starting from regions where they were in reasonable agreement to produce an improved model which yielded an R value of 0.169 for 12 816 observed reflections in the 10-2 A resolution range. The reconciled model was compared with the structure of the 88% relative humidity form obtained through a water-mediated transformation [Madhusudan, Kodandapani & Vijayan (1993). Acta Cryst. D49, 234-245]. Parts of the flexible regions of the molecule register significant movements during the transformation. The changes resulting from the transformation from the native to the low-humidity forms are pronounced in many of the side chains in the active-site region, thus indicating the relationship between hydration, mobility and enzyme action. The fact that the overall changes in molecular geometry resulting from water-mediated transformation are similar to those which occur during enzyme action, further emphasizes this relationship.
About this Structure
1UCO is a Single protein structure of sequence from Gallus gallus. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
An X-ray analysis of native monoclinic lysozyme. A case study on the reliability of refined protein structures and a comparison with the low-humidity form in relation to mobility and enzyme action., Nagendra HG, Sudarsanakumar C, Vijayan M, Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1067-74. PMID:15299565
Page seeded by OCA on Thu Feb 21 15:23:02 2008
