1ujz
From Proteopedia
(New page: 200px<br /><applet load="1ujz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ujz, resolution 2.10Å" /> '''Crystal structure of...) |
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| - | [[Image:1ujz.gif|left|200px]]<br /><applet load="1ujz" size=" | + | [[Image:1ujz.gif|left|200px]]<br /><applet load="1ujz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ujz, resolution 2.10Å" /> | caption="1ujz, resolution 2.10Å" /> | ||
'''Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein'''<br /> | '''Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We developed a 'computational second-site suppressor' strategy to redesign | + | We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show that the designed interfaces are specific in the natural functional context in living cells, and present the first high-resolution X-ray crystallographic analysis of a computer-redesigned functional protein-protein interface with altered specificity. The approach should be applicable to the design of interacting protein pairs with novel specificities for delineating and re-engineering protein interaction networks in living cells. |
==About this Structure== | ==About this Structure== | ||
| - | 1UJZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1UJZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Baker, D.]] | [[Category: Baker, D.]] | ||
| - | [[Category: Bullock, A | + | [[Category: Bullock, A N.]] |
| - | [[Category: Joachimiak, L | + | [[Category: Joachimiak, L A.]] |
[[Category: Kortemme, T.]] | [[Category: Kortemme, T.]] | ||
| - | [[Category: Schuler, A | + | [[Category: Schuler, A D.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: computational design]] | [[Category: computational design]] | ||
[[Category: molecular recognition]] | [[Category: molecular recognition]] | ||
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[[Category: specificity]] | [[Category: specificity]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:18 2008'' |
Revision as of 13:25, 21 February 2008
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Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein
Overview
We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show that the designed interfaces are specific in the natural functional context in living cells, and present the first high-resolution X-ray crystallographic analysis of a computer-redesigned functional protein-protein interface with altered specificity. The approach should be applicable to the design of interacting protein pairs with novel specificities for delineating and re-engineering protein interaction networks in living cells.
About this Structure
1UJZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Computational redesign of protein-protein interaction specificity., Kortemme T, Joachimiak LA, Bullock AN, Schuler AD, Stoddard BL, Baker D, Nat Struct Mol Biol. 2004 Apr;11(4):371-9. Epub 2004 Mar 21. PMID:15034550
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