This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1wa3
From Proteopedia
(New page: 200px<br /> <applet load="1wa3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wa3, resolution 1.90Å" /> '''MECHANISM OF THE CL...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1WA3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with SO4 and PYR as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.14 4.1.2.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WA3 OCA]]. | + | 1WA3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with SO4 and PYR as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/2-dehydro-3-deoxy-phosphogluconate_aldolase 2-dehydro-3-deoxy-phosphogluconate aldolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.14 4.1.2.14]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WA3 OCA]]. |
==Reference== | ==Reference== | ||
Mechanism of the Class I KDPG aldolase., Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH, Bioorg Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403639 16403639] | Mechanism of the Class I KDPG aldolase., Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH, Bioorg Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403639 16403639] | ||
| + | [[Category: 2-dehydro-3-deoxy-phosphogluconate aldolase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
| Line 30: | Line 31: | ||
[[Category: thermotoga maritima]] | [[Category: thermotoga maritima]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:56:30 2007'' |
Revision as of 11:51, 30 October 2007
|
MECHANISM OF THE CLASS I KDPG ALDOLASE
Overview
In vivo, 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase catalyzes the, reversible, stereospecific retro-aldol cleavage of KDPG to pyruvate and, D-glyceraldehyde-3-phosphate. The enzyme is a lysine-dependent (Class I), aldolase that functions through the intermediacy of a Schiff base. Here, we propose a mechanism for this enzyme based on crystallographic studies, of wild-type and mutant aldolases. The three dimensional structure of KDPG, aldolase from the thermophile Thermotoga maritima was determined to 1.9A., The structure is the standard alpha/beta barrel observed for all Class I, aldolases. At the active site Lys we observe clear density for a pyruvate, Schiff base. Density for a sulfate ion bound in a conserved cluster of, residues close to the Schiff base is also observed. We have ... [(full description)]
About this Structure
1WA3 is a [Single protein] structure of sequence from [Thermotoga maritima] with SO4 and PYR as [ligands]. Active as [2-dehydro-3-deoxy-phosphogluconate aldolase], with EC number [4.1.2.14]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Mechanism of the Class I KDPG aldolase., Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH, Bioorg Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PMID:16403639
Page seeded by OCA on Tue Oct 30 13:56:30 2007
