1vg0
From Proteopedia
(New page: 200px<br /><applet load="1vg0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vg0, resolution 2.20Å" /> '''The crystal structur...) |
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| - | [[Image:1vg0.jpg|left|200px]]<br /><applet load="1vg0" size=" | + | [[Image:1vg0.jpg|left|200px]]<br /><applet load="1vg0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vg0, resolution 2.20Å" /> | caption="1vg0, resolution 2.20Å" /> | ||
'''The crystal structures of the REP-1 protein in complex with monoprenylated Rab7 protein'''<br /> | '''The crystal structures of the REP-1 protein in complex with monoprenylated Rab7 protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Members of the RabGDI/REP family serve as multifunctional regulators of | + | Members of the RabGDI/REP family serve as multifunctional regulators of the Rab family of GTP binding proteins. Mutations in members of this family, such as REP-1, lead to abnormalities, including progressive retinal degradation (choroideremia) in humans. The crystal structures of the REP-1 protein in complex with monoprenylated or C-terminally truncated Rab7 proteins revealed that Rab7 interacts with the Rab binding platform of REP-1 via an extended interface involving the Switch 1 and 2 regions. The C terminus of the REP-1 molecule functions as a mobile lid covering a conserved hydrophobic patch on the surface of REP-1 that in the complex coordinates the C terminus of Rab proteins. Using semisynthetic fluorescent Rab27A, we demonstrate that although Rab27A can be prenylated by REP-2, this reaction can be effectively inhibited by other Rab proteins, providing a possible explanation for the accumulation of unprenylated Rab27A in choroideremia. |
==About this Structure== | ==About this Structure== | ||
| - | 1VG0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MG, CL, GER, GDP and PG4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1VG0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=GER:'>GER</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VG0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Alexandrov, K.]] | [[Category: Alexandrov, K.]] | ||
| - | [[Category: Goody, R | + | [[Category: Goody, R S.]] |
[[Category: Niculae, A.]] | [[Category: Niculae, A.]] | ||
[[Category: Pyatkov, K.]] | [[Category: Pyatkov, K.]] | ||
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[[Category: rab prenylation]] | [[Category: rab prenylation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:34:56 2008'' |
Revision as of 13:34, 21 February 2008
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The crystal structures of the REP-1 protein in complex with monoprenylated Rab7 protein
Overview
Members of the RabGDI/REP family serve as multifunctional regulators of the Rab family of GTP binding proteins. Mutations in members of this family, such as REP-1, lead to abnormalities, including progressive retinal degradation (choroideremia) in humans. The crystal structures of the REP-1 protein in complex with monoprenylated or C-terminally truncated Rab7 proteins revealed that Rab7 interacts with the Rab binding platform of REP-1 via an extended interface involving the Switch 1 and 2 regions. The C terminus of the REP-1 molecule functions as a mobile lid covering a conserved hydrophobic patch on the surface of REP-1 that in the complex coordinates the C terminus of Rab proteins. Using semisynthetic fluorescent Rab27A, we demonstrate that although Rab27A can be prenylated by REP-2, this reaction can be effectively inhibited by other Rab proteins, providing a possible explanation for the accumulation of unprenylated Rab27A in choroideremia.
About this Structure
1VG0 is a Protein complex structure of sequences from Rattus norvegicus with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease., Rak A, Pylypenko O, Niculae A, Pyatkov K, Goody RS, Alexandrov K, Cell. 2004 Jun 11;117(6):749-60. PMID:15186776
Page seeded by OCA on Thu Feb 21 15:34:56 2008
Categories: Protein complex | Rattus norvegicus | Alexandrov, K. | Goody, R S. | Niculae, A. | Pyatkov, K. | Pylypenko, O. | Rak, A. | CL | GDP | GER | MG | PG4 | Post-translational modification | Rab prenylation
