1vpw
From Proteopedia
(New page: 200px<br /><applet load="1vpw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vpw, resolution 2.700Å" /> '''STRUCTURE OF THE PU...) |
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| - | [[Image:1vpw.gif|left|200px]]<br /><applet load="1vpw" size=" | + | [[Image:1vpw.gif|left|200px]]<br /><applet load="1vpw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vpw, resolution 2.700Å" /> | caption="1vpw, resolution 2.700Å" /> | ||
'''STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA'''<br /> | '''STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the purine repressor mutant L54M bound to | + | The crystal structure of the purine repressor mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these purine repressors bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C.G:G.C base pair step. Modeling studies, supported by genetic and biochemical data, show that the stereochemistry of the backbone atoms of the abutting hinge helices combined with the rigidity of the kinked base pair step constrain the interdigitating residue to leucine or methionine for the LacI/GalR family of transcription regulators. |
==About this Structure== | ==About this Structure== | ||
| - | 1VPW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1VPW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HPA:'>HPA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VPW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arvidson, R | + | [[Category: Arvidson, R G.Brennan D N.]] |
[[Category: Faber, C.]] | [[Category: Faber, C.]] | ||
[[Category: Lu, F.]] | [[Category: Lu, F.]] | ||
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[[Category: minor groove bending]] | [[Category: minor groove bending]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:26 2008'' |
Revision as of 13:37, 21 February 2008
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STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA
Overview
The crystal structure of the purine repressor mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these purine repressors bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C.G:G.C base pair step. Modeling studies, supported by genetic and biochemical data, show that the stereochemistry of the backbone atoms of the abutting hinge helices combined with the rigidity of the kinked base pair step constrain the interdigitating residue to leucine or methionine for the LacI/GalR family of transcription regulators.
About this Structure
1VPW is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
The structure of PurR mutant L54M shows an alternative route to DNA kinking., Arvidson DN, Lu F, Faber C, Zalkin H, Brennan RG, Nat Struct Biol. 1998 Jun;5(6):436-41. PMID:9628480
Page seeded by OCA on Thu Feb 21 15:37:26 2008
