1waf
From Proteopedia
(New page: 200px<br /><applet load="1waf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1waf, resolution 3.2Å" /> '''DNA POLYMERASE FROM B...) |
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| - | [[Image:1waf.gif|left|200px]]<br /><applet load="1waf" size=" | + | [[Image:1waf.gif|left|200px]]<br /><applet load="1waf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1waf, resolution 3.2Å" /> | caption="1waf, resolution 3.2Å" /> | ||
'''DNA POLYMERASE FROM BACTERIOPHAGE RB69'''<br /> | '''DNA POLYMERASE FROM BACTERIOPHAGE RB69'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a | + | The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases. |
==About this Structure== | ==About this Structure== | ||
| - | 1WAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18] with GMP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1WAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18] with <scene name='pdbligand=GMP:'>GMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage rb18]] | [[Category: Enterobacteria phage rb18]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Karam, J | + | [[Category: Karam, J D.]] |
| - | [[Category: Konigsberg, W | + | [[Category: Konigsberg, W H.]] |
| - | [[Category: Satter, A | + | [[Category: Satter, A K.M A.]] |
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
| - | [[Category: Wang, C | + | [[Category: Wang, C C.]] |
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: GMP]] | [[Category: GMP]] | ||
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[[Category: rb69 dna polymerase (gp43)]] | [[Category: rb69 dna polymerase (gp43)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:10 2008'' |
Revision as of 13:42, 21 February 2008
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DNA POLYMERASE FROM BACTERIOPHAGE RB69
Overview
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.
About this Structure
1WAF is a Single protein structure of sequence from Enterobacteria phage rb18 with as ligand. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
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