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(New page: 200px<br /><applet load="1wav" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wav, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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'''CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN'''<br />
'''CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN'''<br />
==Overview==
==Overview==
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The form-B monoclinic insulin crystal was obtained from the sodium citrate, buffer with 1% zinc chloride, keeping phenolic content between 0.76% and, 1.25%. Its space group is P2(1), cell constants are: a = 4.924 nm, b =, 6.094 nm, c = 4.818 nm, beta = 95.8 degrees. There are 6 insulin molecules, which form a hexamer. The initial phase was obtained by using rotation, function program of X-PLOR program package and molecular packing program, of our laboratory. The molecular model was chosen from 4 zinc bovine, insulin hexamer. After the preliminary refinement by using the, macromolecular rigid body refinement technique, the molecular model was, further refined and adjusted by using the energy-minimizing, stereochemically restrained least-squared refinement on the difference, Fourier maps. The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s., deviations from standard bond length and bond angle are 0.0022 nm and 4.7, degrees, respectively.
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The form-B monoclinic insulin crystal was obtained from the sodium citrate buffer with 1% zinc chloride, keeping phenolic content between 0.76% and 1.25%. Its space group is P2(1), cell constants are: a = 4.924 nm, b = 6.094 nm, c = 4.818 nm, beta = 95.8 degrees. There are 6 insulin molecules which form a hexamer. The initial phase was obtained by using rotation function program of X-PLOR program package and molecular packing program of our laboratory. The molecular model was chosen from 4 zinc bovine insulin hexamer. After the preliminary refinement by using the macromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemically restrained least-squared refinement on the difference Fourier maps. The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s. deviations from standard bond length and bond angle are 0.0022 nm and 4.7 degrees, respectively.
==About this Structure==
==About this Structure==
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1WAV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with ZN and IPH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WAV OCA].
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1WAV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=IPH:'>IPH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAV OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Chang, W.R.]]
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[[Category: Chang, W R.]]
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[[Category: Ding, J.H.]]
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[[Category: Ding, J H.]]
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[[Category: Liang, D.C.]]
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[[Category: Liang, D C.]]
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[[Category: Wan, Z.L.]]
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[[Category: Wan, Z L.]]
[[Category: IPH]]
[[Category: IPH]]
[[Category: ZN]]
[[Category: ZN]]
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[[Category: phenol]]
[[Category: phenol]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:17:13 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:15 2008''

Revision as of 13:42, 21 February 2008

Image:1wav.gif

1wav, resolution 2.5Å

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CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN

Overview

The form-B monoclinic insulin crystal was obtained from the sodium citrate buffer with 1% zinc chloride, keeping phenolic content between 0.76% and 1.25%. Its space group is P2(1), cell constants are: a = 4.924 nm, b = 6.094 nm, c = 4.818 nm, beta = 95.8 degrees. There are 6 insulin molecules which form a hexamer. The initial phase was obtained by using rotation function program of X-PLOR program package and molecular packing program of our laboratory. The molecular model was chosen from 4 zinc bovine insulin hexamer. After the preliminary refinement by using the macromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemically restrained least-squared refinement on the difference Fourier maps. The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s. deviations from standard bond length and bond angle are 0.0022 nm and 4.7 degrees, respectively.

About this Structure

1WAV is a Protein complex structure of sequences from Sus scrofa with and as ligands. Full crystallographic information is available from OCA.

Reference

Molecular replacement study on form-B monoclinic crystal of insulin., Ding J, Wan Z, Chang W, Liang D, Sci China C Life Sci. 1996 Apr;39(2):144-53. PMID:8760462

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