1wbf
From Proteopedia
(New page: 200px<br /><applet load="1wbf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wbf, resolution 2.30Å" /> '''WINGED BEAN LECTIN, ...) |
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| - | [[Image:1wbf.jpg|left|200px]]<br /><applet load="1wbf" size=" | + | [[Image:1wbf.jpg|left|200px]]<br /><applet load="1wbf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wbf, resolution 2.30Å" /> | caption="1wbf, resolution 2.30Å" /> | ||
'''WINGED BEAN LECTIN, SACCHARIDE FREE FORM'''<br /> | '''WINGED BEAN LECTIN, SACCHARIDE FREE FORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the saccharide-free form of the basic form of | + | The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop. |
==About this Structure== | ==About this Structure== | ||
| - | 1WBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus] with NAG, MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1WBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Manoj, N.]] | [[Category: Manoj, N.]] | ||
| - | [[Category: Srinivas, V | + | [[Category: Srinivas, V R.]] |
[[Category: Suguna, K.]] | [[Category: Suguna, K.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: saccharide free form]] | [[Category: saccharide free form]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:32 2008'' |
Revision as of 13:42, 21 February 2008
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WINGED BEAN LECTIN, SACCHARIDE FREE FORM
Overview
The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop.
About this Structure
1WBF is a Single protein structure of sequence from Psophocarpus tetragonolobus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of basic winged-bean lectin and a comparison with its saccharide-bound form., Manoj N, Srinivas VR, Suguna K, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310
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