1wwj
From Proteopedia
(New page: 200px<br /><applet load="1wwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wwj, resolution 1.90Å" /> '''crystal structure of...) |
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| - | [[Image:1wwj.gif|left|200px]]<br /><applet load="1wwj" size=" | + | [[Image:1wwj.gif|left|200px]]<br /><applet load="1wwj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wwj, resolution 1.90Å" /> | caption="1wwj, resolution 1.90Å" /> | ||
'''crystal structure of KaiB from Synechocystis sp.'''<br /> | '''crystal structure of KaiB from Synechocystis sp.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cyanobacteria are among the simplest organisms that show daily | + | Cyanobacteria are among the simplest organisms that show daily rhythmicity. Their circadian rhythms consist of the localization, interaction, and accumulation of various proteins, including KaiA, KaiB, KaiC, and SasA. We have determined the 1.9-angstroms resolution crystallographic structure of the cyanobacterial KaiB clock protein from Synechocystis sp. PCC6803. This homotetrameric structure reveals a novel KaiB interface for protein-protein interaction; the protruding hydrophobic helix-turn-helix motif of one subunit fits into a groove between two beta-strands of the adjacent subunit. A cyanobacterial mutant, in which the Asp-Lys salt bridge mediating this tetramer-forming interaction is disrupted by mutation of Asp to Gly, exhibits severely impaired rhythmicity (a short free-running period; approximately 19 h). The KaiB tetramer forms an open square, with positively charged residues around the perimeter. KaiB is localized on the phospholipid-rich membrane and translocates to the cytosol to interact with the other Kai components, KaiA and KaiC. KaiB antagonizes the action of KaiA on KaiC, and shares a sequence-homologous domain with the SasA kinase. Based on our structure, we discuss functional roles for KaiB in the circadian clock. |
==About this Structure== | ==About this Structure== | ||
| - | 1WWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with MLT, IMD and BET as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1WWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=MLT:'>MLT</scene>, <scene name='pdbligand=IMD:'>IMD</scene> and <scene name='pdbligand=BET:'>BET</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechocystis sp.]] | [[Category: Synechocystis sp.]] | ||
| - | [[Category: Arvai, A | + | [[Category: Arvai, A S.]] |
| - | [[Category: Getzoff, E | + | [[Category: Getzoff, E D.]] |
[[Category: Han, S.]] | [[Category: Han, S.]] | ||
[[Category: Hitomi, K.]] | [[Category: Hitomi, K.]] | ||
[[Category: Oyama, T.]] | [[Category: Oyama, T.]] | ||
| - | [[Category: Tainer, J | + | [[Category: Tainer, J A.]] |
[[Category: BET]] | [[Category: BET]] | ||
[[Category: IMD]] | [[Category: IMD]] | ||
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[[Category: clock]] | [[Category: clock]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:48 2008'' |
Revision as of 13:48, 21 February 2008
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crystal structure of KaiB from Synechocystis sp.
Overview
Cyanobacteria are among the simplest organisms that show daily rhythmicity. Their circadian rhythms consist of the localization, interaction, and accumulation of various proteins, including KaiA, KaiB, KaiC, and SasA. We have determined the 1.9-angstroms resolution crystallographic structure of the cyanobacterial KaiB clock protein from Synechocystis sp. PCC6803. This homotetrameric structure reveals a novel KaiB interface for protein-protein interaction; the protruding hydrophobic helix-turn-helix motif of one subunit fits into a groove between two beta-strands of the adjacent subunit. A cyanobacterial mutant, in which the Asp-Lys salt bridge mediating this tetramer-forming interaction is disrupted by mutation of Asp to Gly, exhibits severely impaired rhythmicity (a short free-running period; approximately 19 h). The KaiB tetramer forms an open square, with positively charged residues around the perimeter. KaiB is localized on the phospholipid-rich membrane and translocates to the cytosol to interact with the other Kai components, KaiA and KaiC. KaiB antagonizes the action of KaiA on KaiC, and shares a sequence-homologous domain with the SasA kinase. Based on our structure, we discuss functional roles for KaiB in the circadian clock.
About this Structure
1WWJ is a Single protein structure of sequence from Synechocystis sp. with , and as ligands. Full crystallographic information is available from OCA.
Reference
Tetrameric architecture of the circadian clock protein KaiB. A novel interface for intermolecular interactions and its impact on the circadian rhythm., Hitomi K, Oyama T, Han S, Arvai AS, Getzoff ED, J Biol Chem. 2005 May 13;280(19):19127-35. Epub 2005 Feb 16. PMID:15716274
Page seeded by OCA on Thu Feb 21 15:48:48 2008
Categories: Single protein | Synechocystis sp. | Arvai, A S. | Getzoff, E D. | Han, S. | Hitomi, K. | Oyama, T. | Tainer, J A. | BET | IMD | MLT | Circadian | Clock
