1xm5
From Proteopedia
(New page: 200px<br /><applet load="1xm5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xm5, resolution 2.7Å" /> '''Crystal structure of ...) |
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| - | [[Image:1xm5.jpg|left|200px]]<br /><applet load="1xm5" size=" | + | [[Image:1xm5.jpg|left|200px]]<br /><applet load="1xm5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xm5, resolution 2.7Å" /> | caption="1xm5, resolution 2.7Å" /> | ||
'''Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054'''<br /> | '''Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional crystallographic structure of the ybeY protein from | + | The three-dimensional crystallographic structure of the ybeY protein from Escherichia coli (SwissProt entry P77385) is reported at 2.7 A resolution. YbeY is a hypothetical protein that belongs to the UPF0054 family. The structure reveals that the protein binds a metal ion in a tetrahedral geometry. Three coordination sites are provided by histidine residues, while the fourth might be a water molecule that is not seen in the diffraction map because of its relatively low resolution. X-ray fluorescence analysis of the purified protein suggests that the metal is a nickel ion. The structure of ybeY and its sequence similarity to a number of predicted metal-dependent hydrolases provides a functional assignment for this protein family. The figures and tables of this paper were prepared using semi-automated tools, termed the Autopublish server, developed by the New York Structural GenomiX Research Consortium, with the goal of facilitating the rapid publication of crystallographic structures that emanate from worldwide Structural Genomics efforts, including the NIH-funded Protein Structure Initiative. |
==About this Structure== | ==About this Structure== | ||
| - | 1XM5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1XM5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XM5 OCA]. |
==Reference== | ==Reference== | ||
| - | The ybeY protein from Escherichia coli is a metalloprotein., Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA, Acta | + | The ybeY protein from Escherichia coli is a metalloprotein., Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):959-63. Epub 2005 Oct 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511207 16511207] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Almo, S | + | [[Category: Almo, S C.]] |
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Fedorov, A | + | [[Category: Fedorov, A A.]] |
| - | [[Category: Fedorov, E | + | [[Category: Fedorov, E V.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
| - | [[Category: Ramagopal, U | + | [[Category: Ramagopal, U A.]] |
[[Category: Shi, W.]] | [[Category: Shi, W.]] | ||
[[Category: Thirumuruhan, R.]] | [[Category: Thirumuruhan, R.]] | ||
| Line 29: | Line 29: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:23 2008'' |
Revision as of 13:56, 21 February 2008
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Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054
Overview
The three-dimensional crystallographic structure of the ybeY protein from Escherichia coli (SwissProt entry P77385) is reported at 2.7 A resolution. YbeY is a hypothetical protein that belongs to the UPF0054 family. The structure reveals that the protein binds a metal ion in a tetrahedral geometry. Three coordination sites are provided by histidine residues, while the fourth might be a water molecule that is not seen in the diffraction map because of its relatively low resolution. X-ray fluorescence analysis of the purified protein suggests that the metal is a nickel ion. The structure of ybeY and its sequence similarity to a number of predicted metal-dependent hydrolases provides a functional assignment for this protein family. The figures and tables of this paper were prepared using semi-automated tools, termed the Autopublish server, developed by the New York Structural GenomiX Research Consortium, with the goal of facilitating the rapid publication of crystallographic structures that emanate from worldwide Structural Genomics efforts, including the NIH-funded Protein Structure Initiative.
About this Structure
1XM5 is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
The ybeY protein from Escherichia coli is a metalloprotein., Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):959-63. Epub 2005 Oct 20. PMID:16511207
Page seeded by OCA on Thu Feb 21 15:56:23 2008
Categories: Escherichia coli | Single protein | Almo, S C. | Burley, S K. | Fedorov, A A. | Fedorov, E V. | NYSGXRC, New York Structural GenomiX Research Consortium. | Ramagopal, U A. | Shi, W. | Thirumuruhan, R. | NI | New york structural genomix research consortium | Nysgxrc target t842 | Pfam family upf0054 | Protein structure initiative | Psi | Structural genomics
