1xqp
From Proteopedia
(New page: 200px<br /><applet load="1xqp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xqp, resolution 1.69Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1xqp.jpg|left|200px]]<br /><applet load="1xqp" size=" | + | [[Image:1xqp.jpg|left|200px]]<br /><applet load="1xqp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xqp, resolution 1.69Å" /> | caption="1xqp, resolution 1.69Å" /> | ||
'''Crystal structure of 8-oxoguanosine complexed Pa-AGOG, 8-oxoguanine DNA glycosylase from Pyrobaculum aerophilum'''<br /> | '''Crystal structure of 8-oxoguanosine complexed Pa-AGOG, 8-oxoguanine DNA glycosylase from Pyrobaculum aerophilum'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Studies of DNA base excision repair (BER) pathways in the | + | Studies of DNA base excision repair (BER) pathways in the hyperthermophilic crenarchaeon Pyrobaculum aerophilum identified an 8-oxoguanine-DNA glycosylase, Pa-AGOG (archaeal GO glycosylase), with distinct functional characteristics. Here, we describe its crystal structure and that of its complex with 8-oxoguanosine at 1.0 and 1.7 A resolution, respectively. Characteristic structural features are identified that confirm Pa-AGOG to be the founding member of a functional class within the helix-hairpin-helix (HhH) superfamily of DNA repair enzymes. Its hairpin structure differs substantially from that of other proteins containing an HhH motif, and we predict that it interacts with the DNA backbone in a distinct manner. Furthermore, the mode of 8-oxoguanine recognition, which involves several hydrogen-bonding and pi-stacking interactions, is unlike that observed in human OGG1, the prototypic 8-oxoguanine HhH-type DNA glycosylase. Despite these differences, the predicted kinked conformation of bound DNA and the catalytic mechanism are likely to resemble those of human OGG1. |
==About this Structure== | ==About this Structure== | ||
| - | 1XQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with 8HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1XQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum] with <scene name='pdbligand=8HG:'>8HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XQP OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Jiricny, J.]] | [[Category: Jiricny, J.]] | ||
[[Category: Kostrewa, D.]] | [[Category: Kostrewa, D.]] | ||
| - | [[Category: Lingaraju, G | + | [[Category: Lingaraju, G M.]] |
| - | [[Category: Prota, A | + | [[Category: Prota, A E.]] |
| - | [[Category: Sartori, A | + | [[Category: Sartori, A A.]] |
| - | [[Category: Winkler, F | + | [[Category: Winkler, F K.]] |
[[Category: 8HG]] | [[Category: 8HG]] | ||
[[Category: 8-oxoguanine dna glycosylase]] | [[Category: 8-oxoguanine dna glycosylase]] | ||
| Line 24: | Line 24: | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
[[Category: helix-hairpin-helix]] | [[Category: helix-hairpin-helix]] | ||
| - | [[Category: p | + | [[Category: p aerophilum]] |
[[Category: pa-agog-8-oxoguanosine complex]] | [[Category: pa-agog-8-oxoguanosine complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:57:38 2008'' |
Revision as of 13:57, 21 February 2008
|
Crystal structure of 8-oxoguanosine complexed Pa-AGOG, 8-oxoguanine DNA glycosylase from Pyrobaculum aerophilum
Overview
Studies of DNA base excision repair (BER) pathways in the hyperthermophilic crenarchaeon Pyrobaculum aerophilum identified an 8-oxoguanine-DNA glycosylase, Pa-AGOG (archaeal GO glycosylase), with distinct functional characteristics. Here, we describe its crystal structure and that of its complex with 8-oxoguanosine at 1.0 and 1.7 A resolution, respectively. Characteristic structural features are identified that confirm Pa-AGOG to be the founding member of a functional class within the helix-hairpin-helix (HhH) superfamily of DNA repair enzymes. Its hairpin structure differs substantially from that of other proteins containing an HhH motif, and we predict that it interacts with the DNA backbone in a distinct manner. Furthermore, the mode of 8-oxoguanine recognition, which involves several hydrogen-bonding and pi-stacking interactions, is unlike that observed in human OGG1, the prototypic 8-oxoguanine HhH-type DNA glycosylase. Despite these differences, the predicted kinked conformation of bound DNA and the catalytic mechanism are likely to resemble those of human OGG1.
About this Structure
1XQP is a Single protein structure of sequence from Pyrobaculum aerophilum with as ligand. Full crystallographic information is available from OCA.
Reference
A DNA glycosylase from Pyrobaculum aerophilum with an 8-oxoguanine binding mode and a noncanonical helix-hairpin-helix structure., Lingaraju GM, Sartori AA, Kostrewa D, Prota AE, Jiricny J, Winkler FK, Structure. 2005 Jan;13(1):87-98. PMID:15642264
Page seeded by OCA on Thu Feb 21 15:57:38 2008
