1xvm
From Proteopedia
(New page: 200px<br /><applet load="1xvm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvm, resolution 1.10Å" /> '''Trypsin from Fusariu...) |
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| - | [[Image:1xvm.gif|left|200px]]<br /><applet load="1xvm" size=" | + | [[Image:1xvm.gif|left|200px]]<br /><applet load="1xvm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xvm, resolution 1.10Å" /> | caption="1xvm, resolution 1.10Å" /> | ||
'''Trypsin from Fusarium oxysporum- room temperature to atomic resolution'''<br /> | '''Trypsin from Fusarium oxysporum- room temperature to atomic resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The analysis of anisotropic atomic displacement parameters for the direct | + | The analysis of anisotropic atomic displacement parameters for the direct extraction of functionally relevant motion from X-ray crystal structures of Fusarium oxysporum trypsin is presented. Several atomic resolution structures complexed with inhibitors or substrates and determined at different pH values and temperatures were investigated. The analysis revealed a breathing-like molecular motion conserved across trypsin structures from two organisms and three different crystal forms. Directional motion was observed suggesting a change of the width of the substrate-binding cleft and a change in the length of the specificity pocket. The differences in direction of motion across the structures are dependent on the mode of substrate or inhibitor binding and the chemical environment around the active-site residues. Together with the occurrence of multiple-residue conformers, they reflect spatial rearrangement throughout the deacylation pathway. |
==About this Structure== | ==About this Structure== | ||
| - | 1XVM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http:// | + | 1XVM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Trypsin]] | [[Category: Trypsin]] | ||
| - | [[Category: Lamzin, V | + | [[Category: Lamzin, V S.]] |
[[Category: Schmidt, A.]] | [[Category: Schmidt, A.]] | ||
[[Category: atomic resolution]] | [[Category: atomic resolution]] | ||
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[[Category: room temperature]] | [[Category: room temperature]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:59:06 2008'' |
Revision as of 13:59, 21 February 2008
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Trypsin from Fusarium oxysporum- room temperature to atomic resolution
Overview
The analysis of anisotropic atomic displacement parameters for the direct extraction of functionally relevant motion from X-ray crystal structures of Fusarium oxysporum trypsin is presented. Several atomic resolution structures complexed with inhibitors or substrates and determined at different pH values and temperatures were investigated. The analysis revealed a breathing-like molecular motion conserved across trypsin structures from two organisms and three different crystal forms. Directional motion was observed suggesting a change of the width of the substrate-binding cleft and a change in the length of the specificity pocket. The differences in direction of motion across the structures are dependent on the mode of substrate or inhibitor binding and the chemical environment around the active-site residues. Together with the occurrence of multiple-residue conformers, they reflect spatial rearrangement throughout the deacylation pathway.
About this Structure
1XVM is a Single protein structure of sequence from Fusarium oxysporum. Active as Trypsin, with EC number 3.4.21.4 Full crystallographic information is available from OCA.
Reference
Extraction of functional motion in trypsin crystal structures., Schmidt A, Lamzin VS, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1132-9. Epub 2005, Jul 20. PMID:16041079
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