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2j73
From Proteopedia
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[[Image:2j73.png|left|200px]] | [[Image:2j73.png|left|200px]] | ||
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{{STRUCTURE_2j73| PDB=2j73 | SCENE= }} | {{STRUCTURE_2j73| PDB=2j73 | SCENE= }} | ||
===ALPHA-GLUCAN RCOGNITION BY A FAMILY 41 CARBOHYDRATE-BINDING MODULE FROM THERMOTOGA MARITIMA PULLULANASE PULA=== | ===ALPHA-GLUCAN RCOGNITION BY A FAMILY 41 CARBOHYDRATE-BINDING MODULE FROM THERMOTOGA MARITIMA PULLULANASE PULA=== | ||
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{{ABSTRACT_PUBMED_17095014}} | {{ABSTRACT_PUBMED_17095014}} | ||
==About this Structure== | ==About this Structure== | ||
| - | + | [[2j73]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J73 OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:017095014</ref><references group="xtra"/> |
[[Category: Pullulanase]] | [[Category: Pullulanase]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
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[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:57:49 2009'' | ||
Revision as of 13:41, 7 January 2013
ALPHA-GLUCAN RCOGNITION BY A FAMILY 41 CARBOHYDRATE-BINDING MODULE FROM THERMOTOGA MARITIMA PULLULANASE PULA
Starch recognition by carbohydrate-binding modules (CBMs) is important for the activity of starch-degrading enzymes. The N-terminal family 41 CBM, TmCBM41 (from pullulanase PulA secreted by Thermotoga maritima) was shown to have alpha-glucan binding activity with specificity for alpha-1,4-glucans but was able to tolerate the alpha-1,6-linkages found roughly every three or four glucose units in pullulan. Using X-ray crystallography, the structures were solved for TmCBM41 in an uncomplexed form and in complex with maltotetraose and 6(3)-alpha-D-glucosyl-maltotriose (GM3). Ligand binding was facilitated by stacking interactions between the alpha-faces of the glucose residues and two tryptophan side-chains in the two main subsites of the carbohydrate-binding site. Overall, this mode of starch binding is quite well conserved by other starch-binding modules. The structure in complex with GM3 revealed a third binding subsite with the flexibility to accommodate an alpha-1,4- or an alpha-1,6-linked glucose.
The structural basis of alpha-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritima., van Bueren AL, Boraston AB, J Mol Biol. 2007 Jan 19;365(3):555-60. Epub 2006 Oct 11. PMID:17095014
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2j73 is a 2 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
- van Bueren AL, Boraston AB. The structural basis of alpha-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritima. J Mol Biol. 2007 Jan 19;365(3):555-60. Epub 2006 Oct 11. PMID:17095014 doi:10.1016/j.jmb.2006.10.018
