1y4z
From Proteopedia
(New page: 200px<br /><applet load="1y4z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y4z, resolution 2.00Å" /> '''The crystal structur...) |
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| - | [[Image:1y4z.gif|left|200px]]<br /><applet load="1y4z" size=" | + | [[Image:1y4z.gif|left|200px]]<br /><applet load="1y4z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1y4z, resolution 2.00Å" /> | caption="1y4z, resolution 2.00Å" /> | ||
'''The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol'''<br /> | '''The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in | + | The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane. |
==About this Structure== | ==About this Structure== | ||
| - | 1Y4Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MD1, 6MO, HEM, SF4, F3S, PCI and AGA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http:// | + | 1Y4Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MD1:'>MD1</scene>, <scene name='pdbligand=6MO:'>6MO</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=F3S:'>F3S</scene>, <scene name='pdbligand=PCI:'>PCI</scene> and <scene name='pdbligand=AGA:'>AGA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nitrate reductase]] | [[Category: Nitrate reductase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bertero, M | + | [[Category: Bertero, M G.]] |
[[Category: Blasco, F.]] | [[Category: Blasco, F.]] | ||
[[Category: Boroumand, N.]] | [[Category: Boroumand, N.]] | ||
[[Category: Ginet, N.]] | [[Category: Ginet, N.]] | ||
[[Category: Palak, M.]] | [[Category: Palak, M.]] | ||
| - | [[Category: Rothery, R | + | [[Category: Rothery, R A.]] |
| - | [[Category: Strynadka, N | + | [[Category: Strynadka, N C.J.]] |
| - | [[Category: Weiner, J | + | [[Category: Weiner, J H.]] |
[[Category: 6MO]] | [[Category: 6MO]] | ||
[[Category: AGA]] | [[Category: AGA]] | ||
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[[Category: q-site]] | [[Category: q-site]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:01:58 2008'' |
Revision as of 14:02, 21 February 2008
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The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol
Overview
The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.
About this Structure
1Y4Z is a Protein complex structure of sequences from Escherichia coli with , , , , , and as ligands. Active as Nitrate reductase, with EC number 1.7.99.4 Full crystallographic information is available from OCA.
Reference
Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A., Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC, J Biol Chem. 2005 Apr 15;280(15):14836-43. Epub 2004 Dec 22. PMID:15615728
Page seeded by OCA on Thu Feb 21 16:01:58 2008
Categories: Escherichia coli | Nitrate reductase | Protein complex | Bertero, M G. | Blasco, F. | Boroumand, N. | Ginet, N. | Palak, M. | Rothery, R A. | Strynadka, N C.J. | Weiner, J H. | 6MO | AGA | F3S | HEM | MD1 | PCI | SF4 | Electron transfer | Membrane protein | Nitrate reduction | Q-site
