1yfn
From Proteopedia
(New page: 200px<br /><applet load="1yfn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yfn, resolution 1.80Å" /> '''Versatile modes of p...) |
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| - | [[Image:1yfn.gif|left|200px]]<br /><applet load="1yfn" size=" | + | [[Image:1yfn.gif|left|200px]]<br /><applet load="1yfn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yfn, resolution 1.80Å" /> | caption="1yfn, resolution 1.80Å" /> | ||
'''Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex'''<br /> | '''Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Energy-dependent proteases often rely on adaptor proteins to modulate | + | Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery. |
==About this Structure== | ==About this Structure== | ||
| - | 1YFN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1YFN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YFN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Baker, T | + | [[Category: Baker, T A.]] |
| - | [[Category: Flynn, J | + | [[Category: Flynn, J M.]] |
| - | [[Category: Grant, R | + | [[Category: Grant, R A.]] |
[[Category: Levchenko, I.]] | [[Category: Levchenko, I.]] | ||
| - | [[Category: Sauer, R | + | [[Category: Sauer, R T.]] |
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
[[Category: rsea]] | [[Category: rsea]] | ||
[[Category: sspb]] | [[Category: sspb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:04:49 2008'' |
Revision as of 14:04, 21 February 2008
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Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex
Overview
Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery.
About this Structure
1YFN is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Versatile modes of peptide recognition by the AAA+ adaptor protein SspB., Levchenko I, Grant RA, Flynn JM, Sauer RT, Baker TA, Nat Struct Mol Biol. 2005 Jun;12(6):520-5. Epub 2005 May 8. PMID:15880122
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