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1yk4
From Proteopedia
(New page: 200px<br /><applet load="1yk4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yk4, resolution 0.69Å" /> '''Ultra-high resolutio...) |
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| - | [[Image:1yk4.gif|left|200px]]<br /><applet load="1yk4" size=" | + | [[Image:1yk4.gif|left|200px]]<br /><applet load="1yk4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yk4, resolution 0.69Å" /> | caption="1yk4, resolution 0.69Å" /> | ||
'''Ultra-high resolution structure of Pyrococcus abyssi rubredoxin W4L/R5S'''<br /> | '''Ultra-high resolution structure of Pyrococcus abyssi rubredoxin W4L/R5S'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was | + | The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was solved by direct methods. The model of the air-oxidized protein was refined by partially restrained full-matrix least-squares refinement against intensity data to 0.69 A resolution. This first ultrahigh-resolution structure of a rubredoxin provides very detailed and precise information about the Fe(SCys)(4) centre and its environment, the peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and dynamic disorder, the solvent structure and the electron-density distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of mutants studied by atomic and ultrahigh-resolution X-ray crystallography which are expected to contribute to the understanding of structure-function relationships in iron-sulfur proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1YK4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YK4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with <scene name='pdbligand=FE:'>FE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bonisch, H.]] | [[Category: Bonisch, H.]] | ||
[[Category: Ladenstein, R.]] | [[Category: Ladenstein, R.]] | ||
| - | [[Category: Schmidt, C | + | [[Category: Schmidt, C L.]] |
[[Category: FE]] | [[Category: FE]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:15 2008'' |
Revision as of 14:06, 21 February 2008
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Ultra-high resolution structure of Pyrococcus abyssi rubredoxin W4L/R5S
Overview
The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was solved by direct methods. The model of the air-oxidized protein was refined by partially restrained full-matrix least-squares refinement against intensity data to 0.69 A resolution. This first ultrahigh-resolution structure of a rubredoxin provides very detailed and precise information about the Fe(SCys)(4) centre and its environment, the peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and dynamic disorder, the solvent structure and the electron-density distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of mutants studied by atomic and ultrahigh-resolution X-ray crystallography which are expected to contribute to the understanding of structure-function relationships in iron-sulfur proteins.
About this Structure
1YK4 is a Single protein structure of sequence from Pyrococcus abyssi with as ligand. Full crystallographic information is available from OCA.
Reference
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin. I. 0.69 A X-ray structure of mutant W4L/R5S., Bonisch H, Schmidt CL, Bianco P, Ladenstein R, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):990-1004. Epub 2005, Jun 24. PMID:15983423
Page seeded by OCA on Thu Feb 21 16:06:15 2008
