1ykh
From Proteopedia
(New page: 200px<br /><applet load="1ykh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ykh, resolution 3.0Å" /> '''Structure of the medi...) |
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| - | [[Image:1ykh.gif|left|200px]]<br /><applet load="1ykh" size=" | + | [[Image:1ykh.gif|left|200px]]<br /><applet load="1ykh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ykh, resolution 3.0Å" /> | caption="1ykh, resolution 3.0Å" /> | ||
'''Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex'''<br /> | '''Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Mediator of transcriptional regulation is the central coactivator that | + | The Mediator of transcriptional regulation is the central coactivator that enables a response of RNA polymerase II (Pol II) to activators and repressors. We present the 3.0-A crystal structure of a highly conserved part of the Mediator, the MED7.MED21 (Med7.Srb7) heterodimer. The structure is very extended, spanning one-third of the Mediator length and almost the diameter of Pol II. It shows a four-helix bundle domain and a coiled-coil protrusion connected by a flexible hinge. Four putative protein binding sites on the surface allow for assembly of the Mediator middle module and for binding of the conserved subunit MED6, which is shown to bridge to the Mediator head module. A flexible MED6 bridge and the MED7.MED21 hinge could account for changes in overall Mediator structure upon binding to Pol II or activators. Our results support the idea that transcription regulation involves conformational changes within the general machinery. |
==About this Structure== | ==About this Structure== | ||
| - | 1YKH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1YKH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YKH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: gene regulation]] | [[Category: gene regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:18 2008'' |
Revision as of 14:06, 21 February 2008
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Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex
Overview
The Mediator of transcriptional regulation is the central coactivator that enables a response of RNA polymerase II (Pol II) to activators and repressors. We present the 3.0-A crystal structure of a highly conserved part of the Mediator, the MED7.MED21 (Med7.Srb7) heterodimer. The structure is very extended, spanning one-third of the Mediator length and almost the diameter of Pol II. It shows a four-helix bundle domain and a coiled-coil protrusion connected by a flexible hinge. Four putative protein binding sites on the surface allow for assembly of the Mediator middle module and for binding of the conserved subunit MED6, which is shown to bridge to the Mediator head module. A flexible MED6 bridge and the MED7.MED21 hinge could account for changes in overall Mediator structure upon binding to Pol II or activators. Our results support the idea that transcription regulation involves conformational changes within the general machinery.
About this Structure
1YKH is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer., Baumli S, Hoeppner S, Cramer P, J Biol Chem. 2005 May 6;280(18):18171-8. Epub 2005 Feb 14. PMID:15710619
Page seeded by OCA on Thu Feb 21 16:06:18 2008
