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1yks
From Proteopedia
(New page: 200px<br /><applet load="1yks" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yks, resolution 1.8Å" /> '''Crystal structure of ...) |
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| - | [[Image:1yks.gif|left|200px]]<br /><applet load="1yks" size=" | + | [[Image:1yks.gif|left|200px]]<br /><applet load="1yks" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yks, resolution 1.8Å" /> | caption="1yks, resolution 1.8Å" /> | ||
'''Crystal structure of yellow fever virus NS3 helicase'''<br /> | '''Crystal structure of yellow fever virus NS3 helicase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Yellow fever virus (YFV), a member of the Flavivirus genus, has a | + | Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus replication and to RNA capping. The 1.8-A crystal structure of the helicase region of the YFV NS3 protein includes residues 187 to 623. Two familiar helicase domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates and RNA triphosphate. The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans. |
==About this Structure== | ==About this Structure== | ||
| - | 1YKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Active as [http://en.wikipedia.org/wiki/Flavivirin Flavivirin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.91 3.4.21.91] Full crystallographic information is available from [http:// | + | 1YKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Active as [http://en.wikipedia.org/wiki/Flavivirin Flavivirin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.91 3.4.21.91] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YKS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Yellow fever virus]] | [[Category: Yellow fever virus]] | ||
| - | [[Category: Bera, A | + | [[Category: Bera, A K.]] |
| - | [[Category: Kuhn, R | + | [[Category: Kuhn, R J.]] |
| - | [[Category: Smith, J | + | [[Category: Smith, J L.]] |
[[Category: Wu, J.]] | [[Category: Wu, J.]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
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[[Category: yellow fever virus]] | [[Category: yellow fever virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:21 2008'' |
Revision as of 14:06, 21 February 2008
|
Crystal structure of yellow fever virus NS3 helicase
Overview
Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus replication and to RNA capping. The 1.8-A crystal structure of the helicase region of the YFV NS3 protein includes residues 187 to 623. Two familiar helicase domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates and RNA triphosphate. The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans.
About this Structure
1YKS is a Single protein structure of sequence from Yellow fever virus. Active as Flavivirin, with EC number 3.4.21.91 Full crystallographic information is available from OCA.
Reference
Structure of the Flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing., Wu J, Bera AK, Kuhn RJ, Smith JL, J Virol. 2005 Aug;79(16):10268-77. PMID:16051820
Page seeded by OCA on Thu Feb 21 16:06:21 2008
Categories: Flavivirin | Single protein | Yellow fever virus | Bera, A K. | Kuhn, R J. | Smith, J L. | Wu, J. | Atpase | Dead-box | Flavivirus | Helicase | Rtpase
