1yvr
From Proteopedia
(New page: 200px<br /><applet load="1yvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yvr, resolution 1.95Å" /> '''Ro autoantigen'''<br...) |
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| - | [[Image:1yvr.gif|left|200px]]<br /><applet load="1yvr" size=" | + | [[Image:1yvr.gif|left|200px]]<br /><applet load="1yvr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1yvr, resolution 1.95Å" /> | caption="1yvr, resolution 1.95Å" /> | ||
'''Ro autoantigen'''<br /> | '''Ro autoantigen'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Ro 60 kDa autoantigen is a major target of the immune response in | + | The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro. |
==About this Structure== | ==About this Structure== | ||
| - | 1YVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http:// | + | 1YVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Fu, C.]] | [[Category: Fu, C.]] | ||
[[Category: Fuchs, G.]] | [[Category: Fuchs, G.]] | ||
| - | [[Category: Reinisch, K | + | [[Category: Reinisch, K M.]] |
| - | [[Category: Stein, A | + | [[Category: Stein, A J.]] |
| - | [[Category: Wolin, S | + | [[Category: Wolin, S L.]] |
[[Category: heat repeat; von willebrand factor a; rossmann fold; midas motif']] | [[Category: heat repeat; von willebrand factor a; rossmann fold; midas motif']] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:35 2008'' |
Revision as of 14:09, 21 February 2008
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Ro autoantigen
Overview
The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.
About this Structure
1YVR is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity., Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM, Cell. 2005 May 20;121(4):529-39. PMID:15907467
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