1z24
From Proteopedia
(New page: 200px<br /><applet load="1z24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z24, resolution 2.6Å" /> '''The molecular structu...) |
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| - | [[Image:1z24.gif|left|200px]]<br /><applet load="1z24" size=" | + | [[Image:1z24.gif|left|200px]]<br /><applet load="1z24" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z24, resolution 2.6Å" /> | caption="1z24, resolution 2.6Å" /> | ||
'''The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.'''<br /> | '''The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Insecticyanin, a blue biliprotein isolated from the tobacco hornworm | + | Insecticyanin, a blue biliprotein isolated from the tobacco hornworm Manduca sexta L., is involved in insect camouflage. Its three-dimensional structure has now been solved to 2.6 A resolution using the techniques of multiple isomorphous replacement, non-crystallographic symmetry averaging about a local 2-fold rotation axis and solvent flattening. All 189 amino acids have been fitted to the electron density map. The map clearly shows that insecticyanin is a tetramer with one of its molecular 2-fold axes coincident to a crystallographic dyad. The individual subunits have overall dimensions of 44 A X 37 A X 40 A and consist primarily of an eight-stranded anti-parallel beta-barrel flanked on one side by a 4.5-turn alpha-helix. Interestingly the overall three-dimensional fold of the insecticyanin subunit shows remarkable similarity to the structural motifs of bovine beta-lactoglobulin and the human serum retinol-binding protein. The electron density attributable to the chromophore is unambiguous and shows that it is indeed the gamma-isomer of biliverdin. The biliverdin lies towards the open end of the beta-barrel with its two propionate side chains pointing towards the solvent and it adopts a rather folded conformation, much like a heme. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta] with BLV as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1Z24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta] with <scene name='pdbligand=BLV:'>BLV</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z24 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Manduca sexta]] | [[Category: Manduca sexta]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Holden, H | + | [[Category: Holden, H M.]] |
| - | [[Category: Law, J | + | [[Category: Law, J H.]] |
[[Category: Rayment, I.]] | [[Category: Rayment, I.]] | ||
| - | [[Category: Rypniewski, W | + | [[Category: Rypniewski, W R.]] |
[[Category: BLV]] | [[Category: BLV]] | ||
[[Category: blue biliprotein]] | [[Category: blue biliprotein]] | ||
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[[Category: ins-a]] | [[Category: ins-a]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:22 2008'' |
Revision as of 14:11, 21 February 2008
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The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.
Overview
Insecticyanin, a blue biliprotein isolated from the tobacco hornworm Manduca sexta L., is involved in insect camouflage. Its three-dimensional structure has now been solved to 2.6 A resolution using the techniques of multiple isomorphous replacement, non-crystallographic symmetry averaging about a local 2-fold rotation axis and solvent flattening. All 189 amino acids have been fitted to the electron density map. The map clearly shows that insecticyanin is a tetramer with one of its molecular 2-fold axes coincident to a crystallographic dyad. The individual subunits have overall dimensions of 44 A X 37 A X 40 A and consist primarily of an eight-stranded anti-parallel beta-barrel flanked on one side by a 4.5-turn alpha-helix. Interestingly the overall three-dimensional fold of the insecticyanin subunit shows remarkable similarity to the structural motifs of bovine beta-lactoglobulin and the human serum retinol-binding protein. The electron density attributable to the chromophore is unambiguous and shows that it is indeed the gamma-isomer of biliverdin. The biliverdin lies towards the open end of the beta-barrel with its two propionate side chains pointing towards the solvent and it adopts a rather folded conformation, much like a heme.
About this Structure
1Z24 is a Single protein structure of sequence from Manduca sexta with as ligand. Full crystallographic information is available from OCA.
Reference
The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution., Holden HM, Rypniewski WR, Law JH, Rayment I, EMBO J. 1987 Jun;6(6):1565-70. PMID:3608987
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