1z4o
From Proteopedia
(New page: 200px<br /><applet load="1z4o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z4o, resolution 1.90Å" /> '''Structure of beta-ph...) |
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| - | [[Image:1z4o.gif|left|200px]]<br /><applet load="1z4o" size=" | + | [[Image:1z4o.gif|left|200px]]<br /><applet load="1z4o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z4o, resolution 1.90Å" /> | caption="1z4o, resolution 1.90Å" /> | ||
'''Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate'''<br /> | '''Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | This communication reports the X-ray crystal structure of the - | + | This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z4O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with MG and GL1 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] Full crystallographic information is available from [http:// | + | 1Z4O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GL1:'>GL1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z4O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Allen, K | + | [[Category: Allen, K N.]] |
[[Category: Dai, J.]] | [[Category: Dai, J.]] | ||
[[Category: Dunaway-Mariano, D.]] | [[Category: Dunaway-Mariano, D.]] | ||
| - | [[Category: Tremblay, L | + | [[Category: Tremblay, L W.]] |
[[Category: Zhang, G.]] | [[Category: Zhang, G.]] | ||
[[Category: GL1]] | [[Category: GL1]] | ||
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[[Category: isomerase]] | [[Category: isomerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:02 2008'' |
Revision as of 14:12, 21 February 2008
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Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate
Overview
This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
About this Structure
1Z4O is a Single protein structure of sequence from Lactococcus lactis with and as ligands. Active as Beta-phosphoglucomutase, with EC number 5.4.2.6 Full crystallographic information is available from OCA.
Reference
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase., Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN, J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149
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