1z56
From Proteopedia
(New page: 200px<br /><applet load="1z56" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z56, resolution 3.92Å" /> '''Co-Crystal Structure...) |
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| - | [[Image:1z56.gif|left|200px]]<br /><applet load="1z56" size=" | + | [[Image:1z56.gif|left|200px]]<br /><applet load="1z56" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z56, resolution 3.92Å" /> | caption="1z56, resolution 3.92Å" /> | ||
'''Co-Crystal Structure of Lif1p-Lig4p'''<br /> | '''Co-Crystal Structure of Lif1p-Lig4p'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA ligase IV catalyses the final ligation step in the non-homologous | + | DNA ligase IV catalyses the final ligation step in the non-homologous end-joining (NHEJ) DNA repair pathway and requires interaction of the ligase with the Xrcc4 'genome-guardian', an essential NHEJ factor. Here we report the 3.9 A crystal structure of the Saccharomyces cerevisiae Xrcc4 ortholog ligase interacting factor 1 (Lif1p) complexed with the C-terminal BRCT domains of DNA ligase IV (Lig4p). The structure reveals a novel mode of protein recognition by a tandem BRCT repeat, and in addition provides a molecular basis for a human LIG4 syndrome clinical condition. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z56 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] Full crystallographic information is available from [http:// | + | 1Z56 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z56 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Blundell, T | + | [[Category: Blundell, T L.]] |
| - | [[Category: Chirgadze, D | + | [[Category: Chirgadze, D Y.]] |
| - | [[Category: Davies, O | + | [[Category: Davies, O R.]] |
| - | [[Category: Dore, A | + | [[Category: Dore, A S.]] |
[[Category: Furnham, N.]] | [[Category: Furnham, N.]] | ||
| - | [[Category: Jackson, S | + | [[Category: Jackson, S P.]] |
[[Category: Pellegrini, L.]] | [[Category: Pellegrini, L.]] | ||
| - | [[Category: Sibanda, B | + | [[Category: Sibanda, B L.]] |
[[Category: brct]] | [[Category: brct]] | ||
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
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[[Category: xrcc4]] | [[Category: xrcc4]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:10 2008'' |
Revision as of 14:12, 21 February 2008
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Co-Crystal Structure of Lif1p-Lig4p
Overview
DNA ligase IV catalyses the final ligation step in the non-homologous end-joining (NHEJ) DNA repair pathway and requires interaction of the ligase with the Xrcc4 'genome-guardian', an essential NHEJ factor. Here we report the 3.9 A crystal structure of the Saccharomyces cerevisiae Xrcc4 ortholog ligase interacting factor 1 (Lif1p) complexed with the C-terminal BRCT domains of DNA ligase IV (Lig4p). The structure reveals a novel mode of protein recognition by a tandem BRCT repeat, and in addition provides a molecular basis for a human LIG4 syndrome clinical condition.
About this Structure
1Z56 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Active as DNA ligase (ATP), with EC number 6.5.1.1 Full crystallographic information is available from OCA.
Reference
Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode., Dore AS, Furnham N, Davies OR, Sibanda BL, Chirgadze DY, Jackson SP, Pellegrini L, Blundell TL, DNA Repair (Amst). 2006 Mar 7;5(3):362-8. Epub 2006 Jan 18. PMID:16388993
Page seeded by OCA on Thu Feb 21 16:12:10 2008
